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AB225651

重组人Aquaporin 4蛋白(Tagged)

Recombinant Human Aquaporin 4 protein (Tagged)

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Recombinant Human Aquaporin 4 protein (Tagged) is a Human Fragment protein, in the 253 to 323 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

Aquaporin-4, AQP-4, Mercurial-insensitive water channel, WCH4, MIWC, AQP4

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SDS-PAGE - Recombinant Human Aquaporin 4 protein (Tagged) (AB225651)
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Supplier Data

SDS-PAGE - Recombinant Human Aquaporin 4 protein (Tagged) (AB225651)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225651 with 5% enrichment gel and 15% separation gel.

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

6x His tag N-Terminus SUMO tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

P55087

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 2.922% Sodium chloride, 0.315% Tris HCl

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB9512

Anti-Aquaporin 4 antibody [4/18]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [4/18] (AB9512)

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Proteins & Peptides

AB314609

Recombinant Mouse Aqp2 Protein (His Tag)

SDS-PAGE - Recombinant Mouse Aqp2 Protein (His Tag) (AB314609)

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Proteins & Peptides

AB163206

Recombinant Human Transmembrane protein 30A (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Transmembrane protein 30A (GST tag N-Terminus) (AB163206)

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Proteins & Peptides

AB157901

Recombinant Human Aquaporin 7 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Aquaporin 7 protein (GST tag N-Terminus) (AB157901)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"CPDVEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV","proteinLength":"Fragment","predictedMolecularWeight":"24 kDa","actualMolecularWeight":null,"aminoAcidEnd":323,"aminoAcidStart":253,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P55087","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 4 also known as AQP4 functions as a water channel protein that facilitates the transport of water across cell membranes. It is composed of approximately 323 amino acids resulting in a molecular mass of around 34-38 kDa. This target expresses most in the central nervous system especially in the astrocytes of the brain and spinal cord. Other expression sites include skeletal muscle kidneys and lungs. AQP4 is often studied using various tools including anti-aquaporin 4 antibodies and specific reagents like AQP4 Ab and anti-AQP4.
Biological function summary

Water balance and neural activity regulation are significantly influenced by aquaporin 4. This protein forms a part of the orthogonal array of particles (OAPs) in the plasma membrane which enhances its water permeability function. Through its concentration at the blood-brain barrier AQP4 contributes to maintaining brain homeostasis by regulating osmotic gradients and extracellular space ion concentrations. Its expression and activity may affect critical processes like neuroexcitation and neuroprotection.

Pathways

Aquaporin 4 actively engages in critical water transport and blood-brain barrier integrity pathways. The passive yet selective water transport facilitated by AQP4 impacts pathways including the transcellular pathway that helps in maintaining the homeostatic and osmotic balance important for brain function. Furthermore AQP4 acts in relation to other proteins such as aquaporin 1 and connexin 43 indicating its role in gap junction communication within the brain.

Aquaporin 4 is intricately linked to conditions like neuromyelitis optica (NMO) and cerebral edema. In NMO autoantibodies such as anti-AQP4 target the protein causing demyelination and inflammation of the optic nerve and spinal cord. In cases of cerebral edema an imbalance in AQP4 expression can disrupt water homeostasis leading to exacerbated swelling. AQP4's interaction with proteins like myelin basic protein in these diseases underlines its significance in neurodegeneration and inflammatory processes.
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常规信息

功能

Forms a water-specific channel (PubMed : 19383790, PubMed : 7559426, PubMed : 8601457). Plays an important role in brain water homeostasis (PubMed : 37143309). It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).

序列相似性

Belongs to the MIP/aquaporin (TC 1.A.8) family.

翻译后修饰

Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40% (By similarity).. Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.

亚细胞定位

Endosome membrane

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产品实验方案

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靶点信息

Forms a water-specific channel (PubMed : 19383790, PubMed : 7559426, PubMed : 8601457). Plays an important role in brain water homeostasis (PubMed : 37143309). It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).
See full target information AQP4
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