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AB174391

重组人 Apolipo蛋白 A V/APOA5 - BSA and Azide free (denatured) (His tag N-Terminus)

Recombinant Human Apolipoprotein A V/APOA5 - BSA and Azide free (denatured) (His tag N-Terminus)

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Recombinant Human Apolipoprotein A V/APOA5 - BSA and Azide free (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 24 to 366 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

查看别名

RAP3, UNQ411/PRO773, APOA5, Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3

1 Images
SDS-PAGE - Recombinant Human Apolipoprotein A V/APOA5 - BSA and Azide free (denatured) (His tag N-Terminus) (AB174391)
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Supplier Data

SDS-PAGE - Recombinant Human Apolipoprotein A V/APOA5 - BSA and Azide free (denatured) (His tag N-Terminus) (AB174391)

SDS-PAGE analysis Apolipoprotein A V/APOA5 using 3 μg of ab174391 on a 15% gel.

关键信息

纯度

>85% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q6Q788

不含动物源

No

不含载体蛋白

Yes

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at 3 μg/lane</p>" } } }
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产品详情

This product was previously labelled as Apolipoprotein A V
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSRKGFWDYFSQTSGDKGRVEQIHQQKMAREPATLKDSLEQDLNNMNKFLEKLRPLSGSEAPRLPQDPVGMRRQLQEELEEVKARLQPYMAEAHELVGWNLEGLRQQLKPYTMDLMEQVALRVQELQEQLRVVGEDTKAQLLGGVDEAWALLQGLQSRVVHHTGRFKELFHPYAESLVSGIGRHVQELHRSVAPHAPASPARLSRCVQVLSRKLTLKAKALHARIQQNLDQLREELSRAFAGTGTEEGAGPDPQMLSEEVRQRLQAFRQDTYLQIAAFTRAIDQETEEVQQQLAPPPPGHSAFAPEFQQTDSGKVLSKLQARLDDLWEDITHSLHDQGHSHLGDP","proteinLength":"Full Length","predictedMolecularWeight":"41.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":366,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6Q788","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A V also known as APOA5 plays a critical role in lipid metabolism. This protein possesses a molecular weight of approximately 39 kDa and is mainly expressed in the liver. APOA5 modulates the plasma triglyceride levels by enhancing the uptake and clearance of triglyceride-rich lipoproteins. The protein is located at the surface of these lipoproteins facilitating their interaction with receptors and enzymes like lipoprotein lipase.
Biological function summary

APOA5 influences triglyceride metabolism and is part of the HDL particle complex. It interacts directly with lipoprotein lipase to promote the hydrolysis of triglycerides into free fatty acids. This hydrolytic action aids in reducing triglyceride levels in the plasma. APOA5 also indirectly influences very-low-density lipoprotein (VLDL) metabolism reinforcing its role in lipid regulation.

Pathways

APOA5 integrates into the lipoprotein metabolic pathway and the PPAR signaling pathway. In the lipoprotein metabolic pathway APOA5 works with proteins like APOC3 to influence triglyceride homeostasis. This protein is modulated by PPAR-alpha helping control lipid metabolism and energy homeostasis. Its role in these pathways highlights its impact on triglyceride-rich lipoprotein metabolism positioning it as an important player in maintaining lipid balance.

APOA5 strongly associates with hypertriglyceridemia and cardiovascular disease. Variations in the APOA5 gene can lead to abnormal plasma triglyceride levels contributing to the development of hypertriglyceridemia. Additionally APOA5's connection with APOE proteins through lipid metabolism pathways can influence susceptibility to cardiovascular disorders. Understanding the interactions and mutations within APOA5 provides insights into the mechanisms contributing to these disease states.
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特殊说明

形式

Liquid

附加说明

ab174391 is purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20 mM Tris pH 7.5, 2 mM EDTA.

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常规信息

功能

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed : 17326667).

序列相似性

Belongs to the apolipoprotein A1/A4/E family.

翻译后修饰

Phosphorylated by FAM20C in the extracellular medium.

亚细胞定位

Early endosome

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产品实验方案

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靶点信息

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed : 17326667).
See full target information APOA5
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搭配使用产品

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AB177619

Recombinant Human Apolipoprotein CI/Apo-CI (His tag N-Terminus)

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