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AB87625

重组人 AMF 蛋白 (His tag N-Terminus)

Recombinant Human AMF protein (His tag N-Terminus)

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(3 Publications)

Recombinant Human AMF protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

查看别名

Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, D-hexose-6-phosphate anomerase, Glucose-6-phosphate C2-epimerase, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, Sperm antigen 36, AMF, NLK, PGI, PHI, SA-36

2 Images
Western blot - Recombinant Human AMF protein (His tag N-Terminus) (AB87625)
  • WB

Unknown

Western blot - Recombinant Human AMF protein (His tag N-Terminus) (AB87625)

All lanes:

Anti-AMF antibody (<a href='/products/unavailable/amf-antibody-ab86950'>ab86950</a>) at 1 µg/mL

Lane 1:

Western blot - Recombinant Human AMF protein (His tag N-Terminus) (ab87625) at 0.1 µg

Lane 2:

Western blot - Recombinant Human AMF protein (His tag N-Terminus) (ab87625) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 30s

SDS-PAGE - Recombinant Human AMF protein (His tag N-Terminus) (AB87625)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human AMF protein (His tag N-Terminus) (AB87625)

15% SDS-PAGE showing ab87625 at approximately 65kDa (3µg).

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

WB, SDS-PAGE

applications

生物活性

No

访问

P06744

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab87625 can be used as a WB positive control in conjunction with <a href='/products/unavailable/amf-antibody-ab86950'>ab86950</a>.</p>" } } }
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产品详情

This product was previously labelled as Glucose 6 phosphate isomerase
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06744","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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特殊说明

形式

Liquid

附加说明

ab87625 is purified using conventional chromatography techniques.Endotoxin: < 1.0 EU per 1 μg of protein (determined by LAL method).

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常规信息

功能

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed : 28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine : acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed : 11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed : 11004567, PubMed : 3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed : 11004567, PubMed : 3352745).

序列相似性

Belongs to the GPI family.

翻译后修饰

Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.. ISGylated.

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产品实验方案

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靶点信息

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed : 28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine : acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed : 11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed : 11004567, PubMed : 3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed : 11004567, PubMed : 3352745).
See full target information GPI
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 56:1558-1577 PubMed29905912

2018

Oxidation Resistance 1 Modulates Glycolytic Pathways in the Cerebellum via an Interaction with Glucose-6-Phosphate Isomerase.

Applications

Unspecified application

Species

Unspecified reactive species

Mattéa J Finelli,Teresa Paramo,Elisabete Pires,Brent J Ryan,Richard Wade-Martins,Philip C Biggin,James McCullagh,Peter L Oliver

Molecular medicine reports 13:67-74 PubMed26573126

2015

Microfluidics‑based optimization of neuroleukin‑mediated regulation of articular chondrocyte proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Tian,Weiliang Zhong,Yingqiu Zhang,Baosheng Yin,Weiguo Zhang,Han Liu

Arthritis research & therapy 17:100 PubMed25889507

2015

Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Zong,Tianbao Lu,Shasha Fan,Hui Zhang,Ruhan Gong,Lishan Sun,Zhiyan Fu,Lieying Fan
View all publications
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搭配使用产品

Assay Kits

AB171575

Human Glucose-6-Phosphate Isomerase ELISA Kit

SimpleStep

Sandwich ELISA - Human Glucose-6-Phosphate Isomerase ELISA Kit (AB171575)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB313590

Anti-AMF antibody [EPR28372-33] - BSA and Azide free

BOND RX™ Validated|RabMAb|KO Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR28372-33] - BSA and Azide free (AB313590)

  • 0
  • 0
Proteins & Peptides

AB87361

Recombinant Human 14-3-3 zeta protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB87361)

  • 0
  • 0

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