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AB157838

重组人 Alpha Skeletal Muscle Actin 蛋白 (GST tag N-Terminus)

Recombinant Human Alpha Skeletal Muscle Actin protein (GST tag N-Terminus)

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Recombinant Human Alpha Skeletal Muscle Actin protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 377 aa range, expressed in Wheat germ, suitable for WB, ELISA.

查看别名

ACTA, ACTA1, Alpha-actin-1

2 Images
ELISA - Recombinant Human Alpha Skeletal Muscle Actin protein (GST tag N-Terminus) (AB157838)
  • ELISA

Lab

ELISA - Recombinant Human Alpha Skeletal Muscle Actin protein (GST tag N-Terminus) (AB157838)

1 ug per mL of recombinant protein ab157838 and ab157841 were immobilised in PBS on an ELISA plate overnight. After blocking in 5% BSA, primary antibody (ab119952) was added in a concentration range of 0.017-1000 ng per mL.

Pre-absorbed secondary antibody goat anti-rabbit IgG H&L (HRP, ab97080) was used at 1/20000 dilution.

SDS-PAGE - Recombinant Human Alpha Skeletal Muscle Actin protein (GST tag N-Terminus) (AB157838)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Alpha Skeletal Muscle Actin protein (GST tag N-Terminus) (AB157838)

ab157838 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, WB

applications

生物活性

No

访问

P68133

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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搭配使用产品

Proteins & Peptides

AB240844

Recombinant Human beta Actin protein (His tag)

Mass Spectrometry - Recombinant Human beta Actin protein (His tag) (AB240844)

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Proteins & Peptides

AB134555

Recombinant Human alpha smooth muscle Actin protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human alpha smooth muscle Actin protein (His tag N-Terminus) (AB134555)

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  • 0
Primary Antibodies

AB179467

Anti-Actin antibody [EPR16769] - Loading Control

20ul selling size|RabMAb|Recombinant|Lab Essentials

Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16769] - Loading Control (AB179467)

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Proteins & Peptides

AB157841

Recombinant Human gamma Actin protein (GST tag N-Terminus)

Western blot - Recombinant Human gamma Actin protein (GST tag N-Terminus) (AB157841)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg/mL", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":377,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P68133","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Proprietary technique
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Skeletal Muscle Actin also known as Actin Alpha 1 Actin Alpha and Actin Protein is a highly conserved protein important for muscle function. It has a molecular mass of approximately 42 kDa. This protein is expressed in skeletal muscle tissues and is an essential component of muscle cell's cytoskeleton. The actin family to which Alpha Skeletal Muscle Actin belongs is integral for maintaining cell shape integrity and movement. This isoform of actin is especially significant in forming thin filaments in muscle fibers which are essential for muscle contraction.
Biological function summary

Alpha Skeletal Muscle Actin plays an essential role in the contractile apparatus of muscle cells. It exists as part of the actin filament complex interacting closely with myosin motor proteins. This interaction powers the sliding mechanism that enables muscle contraction and relaxation. Alpha-actin is responsible for mechanical stiffness and tension within muscle tissues enabling muscle fibers to withstand physical stress. The protein's structural role in muscle cells makes it indispensable for proper muscle function and muscle fiber stability.

Pathways

Alpha Skeletal Muscle Actin is deeply involved in the cytoskeletal organization and muscle contraction pathways. It is pivotal in the actin-myosin interaction pathway where it binds to troponin and tropomyosin which regulate muscle contraction cycles. Within these pathways the protein ensures coordinated muscle contractions alongside other proteins like myosin heavy chain and tropomyosin. These interactions coordinate muscle contraction maintaining muscle tone and health.

Alpha Skeletal Muscle Actin mutations have a direct link to various myopathies including actin myopathy and nemaline myopathy. These conditions often result in muscle weakness and occasionally muscle degeneration. The protein's malfunction may also connect to cardiomyopathies due to its important role in muscle function. Research suggests that protein interactions with dystrophin another muscle-associated protein might further elucidate its role in these muscle disorders. Actin dysfunction in these conditions highlights the necessity for healthy actin filaments for skeletal muscle integrity and function.
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常规信息

功能

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

序列相似性

Belongs to the actin family.

翻译后修饰

Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.. Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.. Actin, alpha skeletal muscle, intermediate form. N-terminal cleavage of acetylated cysteine of intermediate muscle actin by ACTMAP.. Methylated at His-75 by SETD3.. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

亚细胞定位

Cytoskeleton

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产品实验方案

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靶点信息

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
See full target information ACTA1
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