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重组人ALKBH2蛋白(ab105622)

Submit a review Q&A (1)References (1)
SDS-PAGE - Recombinant Human ALKBH2 protein (ab105622)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    描述

    • 产品名称

      重组人ALKBH2蛋白

    • 纯度

      > 90 % SDS-PAGE.
      ab105622 was purified using conventional chromatography techniques.

    • 表达系统

      Escherichia coli

    • Accession

      Q6NS38

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        MGSSHHHHHHSSGLVPRGSHMDRFLVKGAQGGLLRKQEEQEPTGEEPAVL GGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADE IFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLS PKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAP GSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPT NTHWYHSLPVRKKVLAPRVNLTFRKILLTKK

      • 预测分子量

        31 kDa including tags

      • 氨基酸

        1 to 261

      • 标签

        His tag N-Terminus

    技术指标

    Our Abpromise guarantee covers the use of ab105622 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      SDS-PAGE

      Mass Spectrometry

    • 质谱法

      MALDI-TOF

    • 形式

      Liquid

    • 补充说明

      Previously labelled as ABH2. 

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.0154% DTT, 0.316% Tris HCl, 30% Glycerol (glycerin, glycerine)

    常规信息

    • 别名

      • 2OG Fe(II) oxy DC1
      • 9530023G02
      • ABH 2
      • ABH2
      • alkB alkylation repair homolog 2
      • ALKB2_HUMAN
      • ALKBH2
      • ALKBH2 alkB
      • Alkylated DNA repair protein alkB homolog
      • Alkylated DNA repair protein alkB homolog 2
      • Alkylation repair homolog 2
      • Alpha ketoglutarate dependent dioxygenase alkB homolog 2
      • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2
      • AU016977
      • DNA oxidative demethylase ALKBH2
      • EC 1.14.11.
      • FLJ99103
      • hABH2
      • mABH2
      • MGC90512
      • Oxy DC1
      see all

    • 功能

      Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.

    • 组织特异性

      Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.

    • 序列相似性

      Belongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.

    • 细胞定位

      Nucleus. Detected in replication foci during S-phase.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human ALKBH2 protein (ab105622)
      SDS-PAGE - Recombinant Human ALKBH2 protein (ab105622)

      15% SDS-PAGE analysis of ab105622 (3µg)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab105622？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab105622 被引用在 1 文献中.

    • Li A  et al. Boosting 5-ALA-based photodynamic therapy by a liposomal nanomedicine through intracellular iron ion regulation. Acta Pharm Sin B 11:1329-1340 (2021). PubMed: 34094837

    客户评价及客户问答

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    Question

    This ABH2 protein lost activity after being stored at -20C for around 8 months.

    Read More
    Answer

    Thank you for your call today and letting us know about the trouble with ab105622.

    As we discussed, I'd be happy to give you a 20% discount on another vial of ab105622 or alternatively you can participate in our testing discount program. In this testing program, you would need to submit a review of ab105622 in a functional assay, and in exchange we will give you one free protein on a future order within the next 4 months.

    Please let me know which of these options you would like, and I will set it up promptly. Let me know if you have any further questions or if there is anything else that we can do for you.

    Read More

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