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AB157880

重组人 Alkaline Phosphatase 蛋白 (GST tag N-Terminus)

Recombinant Human Alkaline Phosphatase protein (GST tag N-Terminus)

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Recombinant Human Alkaline Phosphatase protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 532 aa range, expressed in Wheat germ, suitable for ELISA, WB.

查看别名

Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI

1 Images
SDS-PAGE - Recombinant Human Alkaline Phosphatase protein (GST tag N-Terminus) (AB157880)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human Alkaline Phosphatase protein (GST tag N-Terminus) (AB157880)

ab157880 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, WB

applications

生物活性

No

访问

P09923

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MQGPWVLLLLGLRLQLSLGIIPVEEENPDFWNRQAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTDAAHPGPSVVPALLPLLAGTLLLLGTATAP","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":532,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P09923","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alkaline Phosphatase also known as ALP or ALKPHOS is an enzyme that removes phosphate groups from various molecules like nucleotides and proteins. This enzymatic activity requires alkaline conditions with a pH optimum around 8–10. ALP is anchored to the cell membrane and exists in several isoforms each with a mass around 57-70 kDa. Different tissues express specific isoforms such as bone liver kidney and placenta. Osteoblasts for example show high levels of alkaline phosphatase expression often detected by techniques like ALP staining or alkaline phosphatase staining.
Biological function summary

Alkaline phosphatase plays important roles in dephosphorylation processes. Particularly in bone it is associated with osteoblast activity where it is important for bone mineralization. The enzyme is not known to be part of a large protein complex but its activity aids in the conversion of inorganic pyrophosphate into phosphate which promotes the deposition of calcium phosphate in bone. ALP staining and ALP staining osteoblast can be used in laboratory settings for studying the mineralization and function of osteoblasts.

Pathways

Alkaline phosphatase is key in the regulation of the phosphate group levels within the organism particularly in the bone mineralization process. It participates in the vitamin D metabolic pathway which influences calcium and phosphorus homeostasis. Its function has a dynamic relationship with proteins like NPP1 which regulates levels of inorganic pyrophosphate acting counter to alkaline phosphatase's phosphate-producing activity.

Defects in alkaline phosphatase activity are linked to conditions like hypophosphatasia and hyperphosphatasia where mineralization processes are disrupted. In hypophosphatasia decreased ALP activity leads to improper bone formation due to accumulation of inorganic pyrophosphate. This interaction is notable alongside proteins such as tissular nonspecific alkaline phosphatase. On the other hand elevated ALP levels often indicate liver or bone disease pathology where increased enzyme levels reflect altered tissue turnover.
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特殊说明

形式

Liquid

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常规信息

功能

Alkaline phosphatase that can hydrolyze various phosphate compounds.

序列相似性

Belongs to the alkaline phosphatase family.

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产品实验方案

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靶点信息

Alkaline phosphatase that can hydrolyze various phosphate compounds.
See full target information ALPI
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搭配使用产品

Proteins & Peptides

AB276514

Recombinant Human Alkaline phosphatase protein (His tag)

SDS-PAGE - Recombinant Human Alkaline phosphatase protein (His tag) (AB276514)

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Proteins & Peptides

AB316533

Recombinant Human Placental alkaline phosphatase (PLAP) Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Placental alkaline phosphatase (PLAP) Protein Standard (His tag) (AB316533)

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Primary Antibodies

AB252640

Anti-Alkaline Phosphatase antibody [EPR20428-111] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Alkaline Phosphatase antibody [EPR20428-111] - BSA and Azide free (Detector) (AB252640)

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Assay Kits

AB83369

Alkaline Phosphatase Assay Kit (Colorimetric)

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

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