重组人Alanine Transaminase蛋白(ab113862)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
描述
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产品名称
重组人Alanine Transaminase蛋白
参阅全部 Alanine Transaminase 蛋白酶 -
纯度
> 90 % SDS-PAGE.
ab113862 was purified using conventional chromatography. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHHSSGLVPRGSHMASSTGDRSQAVRHGLRAKVLTLDGMNPRV RRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPIT FLRQVLALCVNPDLLSSPNFPDDAKKRAERILQACGGHSLGAYSVSSGIQ LIREDVARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRT GVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARD HCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVY AAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVV NMDAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDPSFAQFQAEKQ AVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQLPPRAVERAQE LGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLL LEKLSRFHAKFTLEYS -
预测分子量
57 kDa including tags -
氨基酸
1 to 496 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab113862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Mass Spectrometry
SDS-PAGE
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质谱法
MALDI-TOF -
形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.03% DTT, 0.32% Tris HCl, 20% Glycerol
常规信息
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别名
- AAT1
- Alanine aminotransferase
- Alanine aminotransferase 1
see all -
功能
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. -
组织特异性
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue. -
通路
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1. -
序列相似性
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human Alanine Transaminase protein (ab113862)15% SDS-PAGE analysis of ab113862 (3ug)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab113862 尚未被引用在任何文献中。