重组人AKT2蛋白(ab79798)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
描述
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产品名称
重组人AKT2蛋白
参阅全部 AKT2 蛋白酶 -
生物活性
Specific Activity: 5.9 pmol/min/µg. -
纯度
> 90 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLP PLNNFSVAEC QLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREE WMRAIQMVANSLKQRAPGEDPM DYKCGSPSDSSTTEEMEVAVSKARAK VTMNDFDYLKLLGKGTFGKVILVREKATGRYYAM KILRKEVIIAKDEV AHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFH LS RERVFTEERARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKIT DFGLCKEG ISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVM YEMMCGRLPFYNQDHERLFE LILMEEIRFPRTLSPEAKSLLAGLLKKD PKQRLGGGPSDAKEVMEHRFFLSINWQDVVQK KLLPPFKPQVTSEVDT RYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIR E -
氨基酸
1 to 481 -
标签
His tag N-Terminus -
额外的序列信息
Recombinant full-length human AKT2 was co-expressed with PDK1 by baculovirus infection in Sf9 insect cells.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab79798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.1% Imidazole
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, Potassium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- AKT
- Akt2
- AKT2_HUMAN
see all -
功能
General protein kinase capable of phosphorylating several known proteins. -
组织特异性
Expressed in all human cell types so far analyzed. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
图片
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Western blot - Recombinant human AKT2 protein (ab79798)
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SDS-PAGE - Recombinant human AKT2 protein (ab79798)10% SDS-PAGE showing ab79798 at approximately 57kDa (10µg).
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Functional Studies - Recombinant human AKT2 protein (ab79798)Kinase assay: Specific activity 5.9 pmol/min/μg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab79798 被引用在 2 文献中.
- Pan L et al. Inflammatory cytokine-regulated tRNA-derived fragment tRF-21 suppresses pancreatic ductal adenocarcinoma progression. J Clin Invest 131:N/A (2021). PubMed: 34779408
- Liu N et al. Chk1 phosphorylates the tumour suppressor Mig-6, regulating the activation of EGF signalling. EMBO J 31:2365-77 (2012). PubMed: 22505024