重组人AKT2蛋白(ab60322)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人AKT2蛋白
参阅全部 AKT2 蛋白酶 -
纯度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab204851 (AKT peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- AKT
- Akt2
- AKT2_HUMAN
see all -
功能
General protein kinase capable of phosphorylating several known proteins. -
组织特异性
Expressed in all human cell types so far analyzed. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
图片
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Functional Studies - Recombinant human AKT2 protein (ab60322)The specific activity of AKT2 (ab60322) was determined to be 80 nmol/min/mg as per activity assay protocol and was equivalent to 52 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human AKT2 protein (ab60322)SDS PAGE analysis of ab60322 -
SDS-PAGE - Recombinant human AKT2 protein (ab60322)SDS PAGE analysis of ab60322 -
Functional Studies - Recombinant human AKT2 protein (ab60322)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human AKT2 protein (ab60322)ab60322 on SDS-PAGE, MW ~85kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60322 尚未被引用在任何文献中。