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AB93634

重组人 AKR7A2 蛋白 (His tag N-Terminus)

Recombinant Human AKR7A2 protein (His tag N-Terminus)

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Recombinant Human AKR7A2 protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

查看别名

AFAR, AFAR1, AKR7, AKR7A2, Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, Aldoketoreductase 7, Succinic semialdehyde reductase, AFB1-AR 1, SSA reductase

1 Images
SDS-PAGE - Recombinant Human AKR7A2 protein (His tag N-Terminus) (AB93634)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human AKR7A2 protein (His tag N-Terminus) (AB93634)

15% SDS-PAGE showing ab93634 at approximately 44kDa (3μg).

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

WB, SDS-PAGE

applications

生物活性

Yes

生物学活性

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

访问

O43488

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSELEMLSAASRVVSRAAVHCALRSPPPEARALAMSRPPPPRVASVLGTMEMGRRMDAPASAAAVRAFLERGHTELDTAFMYSDGQSETILGGLGLGLGGGDCRVKIATKANPWDGKSLKPDSVRSQLETSLKRLQCPQVDLFYLHAPDHGTPVEETLHACQRLHQEGKFVELGLSNYASWEVAEICTLCKSNGWILPTVYQGMYNATTRQVETELFPCLRHFGLRFYAYNPLAGGLLTGKYKYEDKDGKQPVGRFFGNSWAETYRNRFWKEHHFEAIALVEKALQAAYGASAPSVTSAALRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAATEEGPLEPAVVDAFNQAWHLVAHECPNYFR","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43488","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AKR7A2 also known as aflatoxin aldehyde reductase or succinic semialdehyde reductase is an enzyme with a molecular mass of approximately 38 kDa. It is part of the aldo-keto reductase superfamily and catalyzes the reduction of aldehydes and ketones to their corresponding alcohols using NAD(P)H as a cofactor. AKR7A2 is expressed in various tissues with significant levels in the liver kidney and lungs indicating its involvement in detoxification processes.
Biological function summary

AKR7A2 participates in the detoxification of harmful aldehydes and builds up resistance against oxidative stress. This enzymatic activity helps in converting reactive aldehydes which are formed during lipid peroxidation into less harmful compounds. AKR7A2 does not form part of a larger enzymatic complex but interacts with other molecules within the cell to fulfill its protective roles. By metabolizing these aldehydes AKR7A2 contributes to safeguarding cellular integrity and function.

Pathways

AKR7A2 is involved in cellular defense mechanisms and detoxification pathways. It plays a role in the glutathione metabolism pathway important for handling oxidative damage in cells. AKR7A2 also intersects with pathobiological pathways related to aldehyde detoxification. Its activity correlates with proteins such as glutathione S-transferases (GSTs) which together help neutralize and excrete toxic substances from the body maintaining cellular health.

AKR7A2 has links to cancer and neurodegenerative diseases. High levels of this enzyme are associated with increased capacity to detoxify carcinogenic aldehydes which could contribute to resistance against chemotherapy in certain cancers. Additionally alterations in AKR7A2 activity are seen in neurodegenerative disorders due to its pivotal role in protecting neurons from oxidative stress. Proteins like aldehyde dehydrogenases (ALDHs) also participate in these oxidative pathways and have connections with AKR7A2 in such disease contexts highlighting the enzyme's importance in disease prevention and progression.
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特殊说明

形式

Liquid

附加说明

ab93634 is purified using conventional chromatography techniques.

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常规信息

功能

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.

序列相似性

Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.

亚细胞定位

Mitochondrion

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产品实验方案

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靶点信息

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
See full target information AKR7A2
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搭配使用产品

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