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AB276516

重组人AFM蛋白(His tag)

Recombinant Human AFM protein (His tag)

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Recombinant Human AFM protein (His tag) is a Human Full Length protein, in the 1 to 599 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

查看别名

ALB2, ALBA, AFM, Afamin, Alpha-albumin, Alpha-Alb

1 Images
SDS-PAGE - Recombinant Human AFM protein (His tag) (AB276516)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human AFM protein (His tag) (AB276516)

SDS-PAGE analysis of ab276516

关键信息

纯度

>95% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

HEK 293 cells

标签

His tag C-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

P43652

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.4 Constituents: PBS

storage-buffer

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

序列信息

[{"linker":null,"sequence":"MKLLKLTGFIFFLFFLTESLTLPTQPRDIENFNSTQKFIEDNIEYITIIAFAQYVQEATFEEMEKLVKDMVEYKDRCMADKTLPECSKLPNNVLQEKICAMEGLPQKHNFSHCCSKVDAQRRLCFFYNKKSDVGFLPPFPTLDPEEKCQAYESNRESLLNHFLYEVARRNPFVFAPTLLTVAVHFEEVAKSCCEEQNKVNCLQTRAIPVTQYLKAFSSYQKHVCGALLKFGTKVVHFIYIAILSQKFPKIEFKELISLVEDVSSNYDGCCEGDVVQCIRDTSKVMNHICSKQDSISSKIKECCEKKIPERGQCIINSNKDDRPKDLSLREGKFTDSENVCQERDADPDTFFAKFTFEYSRRHPDLSIPELLRIVQIYKDLLRNCCNTENPPGCYRYAEDKFNETTEKSLKMVQQECKHFQNLGKDGLKYHYLIRLTKIAPQLSTEELVSLGEKMVTAFTTCCTLSEEFACVDNLADLVFGELCGVNENRTINPAVDHCCKTNFAFRRPCFESLKADKTYVPPPFSQDLFTFHADMCQSQNEELQRKTDRFLVNLVKLKHELTDEELQSLFTNFANVVDKCCKAESPEVCFNEESPKIGN","proteinLength":"Full Length","predictedMolecularWeight":"68 kDa","actualMolecularWeight":null,"aminoAcidEnd":599,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P43652","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

性能和储存信息

形式
Lyophilized
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AFM also known as Afamin is a glycoprotein assigned to the albumin family. Its molecular mass is approximately 87 kDa. This protein is mainly expressed in the liver and secreted into the bloodstream. It possesses binding affinity for vitamin E which suggests a role in transport and metabolic processes. It shares structural similarities with other proteins in the albumin family contributing to its functional versatility.
Biological function summary

AFM plays a central role in the transport of lipids and vitamins particularly vitamin E throughout the body. It acts independently and does not participate in large protein complexes. AFM shows interactions with other lipid-binding proteins modulating their activities and ensuring efficient distribution of essential nutrients. Such interactions highlight the importance of AFM in maintaining cellular homeostasis and protecting cells from oxidative damage.

Pathways

AFM actively participates in metabolic and vitamin transport pathways collaborating with proteins like albumin and transthyretin. It notably influences lipid metabolism and antioxidative pathways thereby contributing to overall cellular defense mechanisms. AFM's functionality intertwines with the activities of these proteins indicating its essential role in finely tuned biological processes especially those involving lipid and vitamin management.

AFM has associations with metabolic and neurodegenerative conditions. Altered levels of AFM can correlate with diseases like type 2 diabetes and Alzheimer's disease. It interacts with other relevant proteins such as apolipoproteins impacting disease progression and symptomatology. These connections suggest that AFM could potentially be a biomarker or therapeutic target for managing such disorders opening avenues for future research and treatment strategies.

常规信息

功能

Functions as a carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (PubMed : 26902720). Binds vitamin E (PubMed : 12463752, PubMed : 15952736). May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient (PubMed : 15952736). May be involved in the transport of vitamin E across the blood-brain barrier (PubMed : 19046407).

序列相似性

Belongs to the ALB/AFP/VDB family.

翻译后修饰

N-glycosylated; more than 90% of the glycans are sialylated.

产品实验方案

靶点信息

Functions as a carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (PubMed : 26902720). Binds vitamin E (PubMed : 12463752, PubMed : 15952736). May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient (PubMed : 15952736). May be involved in the transport of vitamin E across the blood-brain barrier (PubMed : 19046407).
See full target information AFM

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