重组人ADAMTS5蛋白(ab134432)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: >= 50% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay
描述
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产品名称
重组人ADAMTS5蛋白 -
生物活性
Aggrecanase activity of ab134432 is determined with recombinant aggrecan interglobular domain. ADAMTS4 hydrolyzes the aggrecanase site within this domain (peptide bond E373-A374 in Human aggrecan). The recombinant substrate is incubated at a concentration of 0.1 µM with ADAMTS5 in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 M CaCl2, 1 µM leupeptin, 1 µM pepstatin, 1 mM Pefabloc, 0.05 % Brij 35 for 15 min at 37°C. A series of ADAMTS5 dilutions ranging from 103 - to 105 -fold is tested. Substrate cleavage at the aggrecanase-site is estimated from the appearance of the hydrolysis fragment with the novel N terminus ARGSVIL. The fragment is quantified with two monoclonal antibodies, one directed against the neoepitope ARGSVIL, the other against the sequence C terminal from the neoepitope. Under the specified conditions the hydrolysis rate is > 0.5 nmoles hydrolyzed substrate/ min x ml ADAMTS5 preparation or > 5 nmoles hydrolyzed substrate/ min x mg.
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纯度
>= 50 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected insect cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
41 kDa including tags -
标签
His tag C-Terminus -
额外的序列信息
Recombinant human ADAMTS5 truncated (the sequence of aminoacids: 625-930 is missing)......
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab134432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
Inhibition Assay
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形式
Liquid -
补充说明
ab134432 contains the catalytic domain, the disintegrin domain and the thrombospondin type 1 motif of full length ADAMTS5.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Preservative: 0.34% Imidazole
Constituents: 0.05% Brij, 0.05% Calcium chloride, 0.79% Tris HCl, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- A disintegrin and metalloproteinase with thrombospondin motifs 11
- A disintegrin and metalloproteinase with thrombospondin motifs 5
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5
see all -
功能
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. -
组织特异性
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. -
序列相似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains. -
结构域
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab134432 尚未被引用在任何文献中。