JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109839

重组人 ADA 蛋白 (His tag N-Terminus)

Recombinant human ADA protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(3 Publications)

Recombinant human ADA protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 363 aa range, expressed in Escherichia coli, with >90%, < 1 EU/mg endotoxin level, suitable for SDS-PAGE, Mass Spec, FuncS.

查看别名

ADA1, ADA, Adenosine deaminase, Adenosine aminohydrolase

1 Images
SDS-PAGE - Recombinant human ADA protein (His tag N-Terminus) (AB109839)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human ADA protein (His tag N-Terminus) (AB109839)

SDS-PAGE analysis of ab109839 at 3μg. Gel concentration : 15%.

关键信息

纯度

>90% SDS-PAGE

No detergents such as urea, Triton or Tween were used to purify the proteins. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA.

内毒素水平

< 1 EU/mg

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

FuncS, Mass Spec, SDS-PAGE

applications

生物活性

Yes

生物学活性

Specific activity is >40 units/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25°C.

访问

P00813

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

搭配使用产品

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

  • 0
  • 0
Proteins & Peptides

AB153069

Recombinant Human TLR6 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human TLR6 protein (GST tag N-Terminus) (AB153069)

  • 0
  • 0
Proteins & Peptides

AB42622

Recombinant human PYK2 protein (His tag N-Terminus)

  • 0
  • 0
Proteins & Peptides

AB152752

Recombinant Human TLR5 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human TLR5 protein (GST tag N-Terminus) (AB152752)

  • 0
  • 0
Biochemicals

AB120297

Phorbol 12-myristate 13-acetate (PMA), PKC activator

Chemical Structure - Phorbol 12-myristate 13-acetate (PMA), PKC activator (AB120297)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>1. Prerapre a 1.5 ml reaction mix: the final concentrations are 53.3mM potassium phosphate, 0.045mM adenosine, 0.003% (w/v) bovine serum.2. Add ab109839 with various concentrations (0.1ug, 0.2) in assay buffer.3. Mix by inversion and record A260nm for approximately 5 minutes.</p>" } } }
style
left-column
style
left-column

序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL","proteinLength":"Full Length","predictedMolecularWeight":"42.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":363,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00813","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine deaminase (ADA) sometimes known as deaminase is an enzyme that plays a critical mechanical role in cellular metabolism. This enzyme catalyzes the deamination of adenosine converting it into inosine. ADA is a 41 kDa protein primarily expressed in most tissues including the thymus and spleen. Various ADA assays and ADA protocols exist to study its functionality and quantification often using ADA ELISA kits to measure ADA activity in biological samples. In cell lines such as HeLa ADA expression is also present and significant for studying its regulation and function.
Biological function summary

Adenosine deaminase activity influences purine metabolism playing an important role in the breakdown of adenosine. It is not part of a complex but interacts closely with other components of the purine salvage pathway to maintain nucleotide balance. This enzyme is important for the proper function of immune cells as it prevents toxic accumulations of adenosine which can be detrimental to lymphocyte proliferation and function. ADA's activity ensures the proper management of nucleotides within cells sustaining cellular health and functionality.

Pathways

Adenosine deaminase plays significant roles in the immune and purine catabolism pathways. Within these pathways ADA is closely related to proteins such as purine nucleoside phosphorylase (PNP) which together orchestrate the steps necessary for adenine and guanine nucleotide turnover. These proteins facilitate the conversion processes needed for cellular nucleic acid synthesis and energy regulation. By managing adenosine levels ADA's function ensures the smooth operation of cellular signaling and metabolic balance.

Adenosine deaminase deficiency is linked to severe combined immunodeficiency (SCID) and can also be associated with pulmonary disorders like asthma. A lack of ADA activity leads to the accumulation of toxic metabolites impairing immune function and contributing to the pathogenesis of SCID. In some patients with pulmonary disorders altered ADA activity impacts the inflammatory response. ADA's mechanistic interactions with other proteins such as PNP highlight its importance in maintaining immune competence and metabolic homeostasis emphasizing the enzyme's role in these clinical conditions.
style
left-column

常规信息

功能

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed : 16670267, PubMed : 23193172, PubMed : 26166670, PubMed : 8452534, PubMed : 9361033). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed : 20959412). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed : 11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed : 20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed : 23193172). Stimulates plasminogen activation (PubMed : 15016824). Plays a role in male fertility (PubMed : 21919946, PubMed : 26166670). Plays a protective role in early postimplantation embryonic development (By similarity). Also responsible for the deamination of cordycepin (3'-deoxyadenosine), a fungal natural product that shows antitumor, antibacterial, antifungal, antivirus, and immune regulation properties (PubMed : 26038697).

序列相似性

Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.

亚细胞定位

Lysosome

style
left-column

产品实验方案

style
left-column

靶点信息

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed : 16670267, PubMed : 23193172, PubMed : 26166670, PubMed : 8452534, PubMed : 9361033). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed : 20959412). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed : 11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed : 20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed : 23193172). Stimulates plasminogen activation (PubMed : 15016824). Plays a role in male fertility (PubMed : 21919946, PubMed : 26166670). Plays a protective role in early postimplantation embryonic development (By similarity). Also responsible for the deamination of cordycepin (3'-deoxyadenosine), a fungal natural product that shows antitumor, antibacterial, antifungal, antivirus, and immune regulation properties (PubMed : 26038697).
See full target information ADA
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 4:109-125 PubMed31909359

2020

Farnesoid X Receptor Agonism, Acetyl-Coenzyme A Carboxylase Inhibition, and Back Translation of Clinically Observed Endpoints of Lipogenesis in a Murine NASH Model.

Applications

Unspecified application

Species

Unspecified reactive species

Berangere Gapp,Marie Jourdain,Pauline Bringer,Benjamin Kueng,Delphine Weber,Arnaud Osmont,Stefan Zurbruegg,Judith Knehr,Rocco Falchetto,Guglielmo Roma,William Dietrich,Reginald Valdez,Nicolau Beckmann,Florian Nigsch,Arun J Sanyal,Iwona Ksiazek

The Biochemical journal 361:203-9 PubMed11772392

2002

Regulation of epithelial and lymphocyte cell adhesion by adenosine deaminase-CD26 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Ginés,Marta Mariño,Josefa Mallol,Enric I Canela,Chikao Morimoto,Christian Callebaut,Ara Hovanessian,Vicent Casadó,Carmen Lluis,Rafael Franco

The Journal of biological chemistry 259:12101-6 PubMed6090454

1984

Human adenosine deaminase. cDNA and complete primary amino acid sequence.

Applications

Unspecified application

Species

Unspecified reactive species

P E Daddona,D S Shewach,W N Kelley,P Argos,A F Markham,S H Orkin
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com