Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
SPECIFIC ACTIVITY: 10,000 – 13,000 units/mg UNIT DEFINATION: One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT
纯度> 95 % SDS-PAGE.
蛋白长度Full length protein
Our Abpromise guarantee covers the use of ab52098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 1082 Caspase-2, human recombinant. 1082-25 is the same size as the 25 unit size of ab52098.
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Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 15% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
- Caspase 2
- ICH 1L
相关性Caspase 2 is involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. During apoptosis, procaspase-2 is processed at aspartate residues. The processed form of caspase-2 consists of large (19 kDa) and small (12 kDa) subunits which associate to form the active enzyme.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.