重组人active Caspase-2蛋白(ab52098)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
描述
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产品名称
重组人active Caspase-2蛋白 -
生物活性
SPECIFIC ACTIVITY: 10,000 – 13,000 units/mg UNIT DEFINATION: One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT
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纯度
> 95 % SDS-PAGE. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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技术指标
Our Abpromise guarantee covers the use of ab52098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
This product is manufactured by BioVision, an Abcam company and was previously called 1082 Caspase-2, human recombinant. 1082-25 is the same size as the 25 unit size of ab52098.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 15% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
常规信息
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别名
- CASP2
- Caspase 2
- ICH 1L
see all -
相关性
Caspase 2 is involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. During apoptosis, procaspase-2 is processed at aspartate residues. The processed form of caspase-2 consists of large (19 kDa) and small (12 kDa) subunits which associate to form the active enzyme. -
细胞定位
Cytosol, membrane
图片
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Western blot - Recombinant human active Caspase-2 protein (ab52098)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab52098 尚未被引用在任何文献中。