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AB114378

重组人 Aconitase 2 蛋白 (GST tag N-Terminus)

Recombinant Human Aconitase 2 protein (GST tag N-Terminus)

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Recombinant Human Aconitase 2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 780 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

查看别名

Aconitase, Citrate hydro-lyase, ACO2

1 Images
SDS-PAGE - Recombinant Human Aconitase 2 protein (GST tag N-Terminus) (AB114378)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Aconitase 2 protein (GST tag N-Terminus) (AB114378)

ab114378 on 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, WB, SDS-PAGE

applications

生物活性

No

访问

Q99798

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MAPYSLLVTRLQKALGVRQYHVASVLCQRAKVAMSHFEPNEYIHYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWKPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWSSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGREDIANLADEFKDHLVPDPGCHYDQLIEINLSELKPHINGPFTPDLAHPVAEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDLGGIVLMAPYSLLVTRLQKALGVRQYHVASVLCQRAKVAMSHFEPNEYIHYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWKPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWSSPKDVILKVAGIL","proteinLength":"Full Length","predictedMolecularWeight":"111.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":780,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q99798","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Aconitase 2 also known as ACO2 is an enzyme involved in the citric acid cycle which plays an important role in energy production within the cell. This enzyme converts citrate to isocitrate via a cis-aconitate intermediate an essential step in the cycle that generates ATP. Aconitase 2 has a molecular mass of approximately 83 kDa and is primarily expressed in the mitochondria of eukaryotic cells where it functions optimally due to the organelle's environment.
Biological function summary

Aconitase 2 supports cellular energy metabolism by facilitating the conversion of citrate derivatives. It is an iron-sulfur protein integral to both the cytosolic aconitase and mitochondrial varieties that functions as part of the mitochondrial aconitase complex. The enzyme's iron-sulfur cluster gets oxidized under different conditions causing the enzyme to act as a regulatory factor in response to iron levels further linking it with cellular iron metabolism.

Pathways

Aconitase 2 plays a significant role in the tricarboxylic acid (TCA) cycle commonly known as the Krebs cycle and the iron regulatory pathway. In the TCA cycle aconitase 2 acts after citrate synthase and before isocitrate dehydrogenase establishing its role in the middle of the cycle. It is functionally related to iron regulatory proteins particularly IRP1 which can interchangeably bind to iron-responsive elements or possess aconitase activity in its Fe-containing form.

Disruptions in aconitase 2 activity are linked to conditions like Friedreich's ataxia and cancer. Friedreich's ataxia involves mitochondrial dysfunction wherein the decreased activity of aconitase 2 leads to impaired energy metabolism contributing to neurodegeneration. In cancers altered aconitase 2 function can impact cellular proliferation by modifying energy metabolism pathways. Aconitase 2 also interacts with the protein frataxin in the context of these conditions underlining its involvement in disease mechanisms related to mitochondrial dysfunction and iron metabolism.
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特殊说明

形式

Liquid

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常规信息

功能

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

序列相似性

Belongs to the aconitase/IPM isomerase family.

翻译后修饰

Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers.

亚细胞定位

Mitochondrion

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产品实验方案

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靶点信息

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
See full target information ACO2
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搭配使用产品

Primary Antibodies

AB110316

Anti-ETFA antibody [2B11AE8]

BOND RX™ Validated|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ETFA antibody [2B11AE8] (AB110316)

  • 0
  • 0
Proteins & Peptides

AB152601

Recombinant Human PDHB protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human PDHB protein (GST tag N-Terminus) (AB152601)

  • 0
  • 0
Primary Antibodies

AB110321

Anti-Aconitase 2 antibody [6F12BD9]

BOND RX™ Validated|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6F12BD9] (AB110321)

  • 5
  • 4
Assay Kits

AB83459

Aconitase Assay Kit

Functional Studies - Aconitase Assay Kit (AB83459)

  • 0
  • 0

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