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AB195158

重组人ACF1 / BAZ1A蛋白

Recombinant Human ACF1 / BAZ1A protein

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Recombinant Human ACF1 / BAZ1A protein is a Human Fragment protein, in the 1415 to 1545 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

ACF1, WCRF180, HSPC317, BAZ1A, Bromodomain adjacent to zinc finger domain protein 1A, ATP-dependent chromatin-remodeling protein, ATP-utilizing chromatin assembly and remodeling factor 1, CHRAC subunit ACF1, Williams syndrome transcription factor-related chromatin-remodeling factor 180, hWALp1, hACF1

1 Images
SDS-PAGE - Recombinant Human ACF1 / BAZ1A protein (AB195158)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ACF1 / BAZ1A protein (AB195158)

4-20% SDS-PAGE using of 2.7 μg ab195158. Coomassie staining.

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

GST tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q9NRL2

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEPSPVTLGRRSSGRQGGVHELSAFEQLVVELVRHDDSWPFLKLVSKIQVPDYYDIIKKPIALNIIREKVNKCEYKLASEFIDDIELMFSNCFEYNPRNTSEAKAGTRLQAFFHIQAQKLGLHVTPSNVDQV","proteinLength":"Fragment","predictedMolecularWeight":"42 kDa","actualMolecularWeight":null,"aminoAcidEnd":1545,"aminoAcidStart":1415,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NRL2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ACF1 also known as BAZ1A is a protein involved in DNA regulation. It is part of the ATP-utilizing chromatin assembly and remodeling factor (ACF) complex. This protein with a molecular mass of around 216 kDa is present mostly in the nucleus. ACF1 shows expression in various tissues including the brain and liver where it operates in regulating chromatin structure and gene accessibility.
Biological function summary

ACF1/BAZ1A is integral to chromatin remodeling which affects how DNA is packaged and accessed by other cellular machinery. It forms part of the ACF complex alongside the ISWI ATPase subunit heavily impacting nucleosome spacing and accessibility. ACF1/BAZ1A assists in nucleosome assembly and the establishment of regular chromatin structures important for gene expression regulation DNA replication and repair processes.

Pathways

ACF1/BAZ1A plays essential roles in pathways related to DNA repair and transcription regulation. In the DNA damage response pathway ACF1/BAZ1A works alongside other proteins like ISWI to ensure proper chromatin architecture facilitating DNA synthesis and repair mechanisms. It also contributes to transcription regulation by altering chromatin structure to allow transcription factors and RNA polymerase II access to DNA.

ACF1/BAZ1A associates with conditions involving genomic instability and cancer. Its role in chromatin remodeling makes it relevant in cancer where faulty chromatin regulation can lead to unchecked cell proliferation. ACF1/BAZ1A has connections with p53 a protein significant in the cellular response to DNA damage further implicating its importance in maintaining genomic integrity. Understanding this protein's role could aid therapeutic strategies targeting chromatin dynamics in these diseases.
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特殊说明

形式

Liquid

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常规信息

功能

Regulatory subunit of the ATP-dependent ACF-1 and ACF-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and slide edge- and center-positioned histone octamers away from their original location on the DNA template to facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed : 17099699, PubMed : 28801535). Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template in an ATP-dependent manner (PubMed : 14759371, PubMed : 17099699, PubMed : 28801535). The ACF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the ACF-5 ISWI chromatin remodeling complex (PubMed : 28801535). Has a role in sensing the length of DNA which flank nucleosomes, which modulates the nucleosome spacing activity of the ACF-5 ISWI chromatin remodeling complex (PubMed : 17099699). Involved in DNA replication and together with SMARCA5/SNF2H is required for replication of pericentric heterochromatin in S-phase (PubMed : 12434153). May have a role in nuclear receptor-mediated transcription repression (PubMed : 17519354).

序列相似性

Belongs to the WAL family.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Regulatory subunit of the ATP-dependent ACF-1 and ACF-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and slide edge- and center-positioned histone octamers away from their original location on the DNA template to facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed : 17099699, PubMed : 28801535). Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template in an ATP-dependent manner (PubMed : 14759371, PubMed : 17099699, PubMed : 28801535). The ACF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the ACF-5 ISWI chromatin remodeling complex (PubMed : 28801535). Has a role in sensing the length of DNA which flank nucleosomes, which modulates the nucleosome spacing activity of the ACF-5 ISWI chromatin remodeling complex (PubMed : 17099699). Involved in DNA replication and together with SMARCA5/SNF2H is required for replication of pericentric heterochromatin in S-phase (PubMed : 12434153). May have a role in nuclear receptor-mediated transcription repression (PubMed : 17519354).
See full target information BAZ1A
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