重组人ACE2 (mutated H374N + H378N)蛋白(Fc Chimera) (ab273885)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% Affinity purified
- Endotoxin level: < 1.000 Eu/mg
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, HPLC, ELISA
描述
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产品名称
重组人ACE2 (mutated H374N + H378N)蛋白(Fc Chimera)
参阅全部 ACE2 蛋白酶 -
纯度
> 95 % Affinity purified. -
内毒素水平
< 1.000 Eu/mg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADGG GGSGGGGSDK THTCPPCPAP EAAGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK -
预测分子量
190 kDa including tags -
氨基酸
18 to 615 -
修饰
mutated H374N + H378N -
标签
Fc tag C-Terminus -
额外的序列信息
Fc domain: Human IgG1 containing silencing LALA mutations.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab273885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
HPLC
ELISA
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形式
Liquid -
补充说明
Recombinant Human ACE2 (mutated H374 N + H378 N) protein (Fc Chimera) (ab273885) is an inactivated form of ACE2 generated from ACE2-Ig by altering the codons of ACE2 active-site histidines 374 and 378 to those of asparagines.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C.
Constituent: PBS
常规信息
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别名
- ACE 2
- ACE related carboxypeptidase
- ACE-related carboxypeptidase
see all -
功能
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
组织特异性
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
序列相似性
Belongs to the peptidase M2 family. -
翻译后修饰
N-glycosylation on Asn-90 may limit SARS infectivity. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
图片
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ELISA - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)
RBD Fc binding to immobilized ACE2 Fc fusion proteins. 96-well plates were coated with Recombinant Human ACE2 protein (Fc Chimera) (ab273687) and ab273885 at 5 µg/ml. The SARS-CoV-2 RBD Fc fusion protein conjugated to HRP was titrated on a 3-fold serial dilution starting at 10 µg/ml.
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SDS-PAGE - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)SDS-PAGE analysis of 2.5 µg ab273885.
Lane 1: Non-reduced.
Lane 2: Reduced.
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HPLC - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)HPLC analysis of ab273885.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab273885 被引用在 1 文献中.
- Kugathasan R et al. Deep mutagenesis scanning using whole trimeric SARS-CoV-2 spike highlights the importance of NTD-RBD interactions in determining spike phenotype. PLoS Pathog 19:e1011545 (2023). PubMed: 37535672
