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重组人14-3-3 Theta + Tau蛋白(ab116444)

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SDS-PAGE - Recombinant Human 14-3-3 Theta + Tau protein (ab116444)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 80% Purified via His tag
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    描述

    • 产品名称

      重组人14-3-3 Theta + Tau蛋白
      参阅全部 14-3-3 Theta + Tau 蛋白酶

    • 纯度

      > 80 % Purified via His tag.
      ab116444 was purified via Ni2+-NTA agarose columns.

    • 表达系统

      Escherichia coli

    • Accession

      P27348

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMEKTELIQKAKLAEQA ERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIE QKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKV FYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPI RLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTL IMQLLRDNLTLWTSDSAGEECDAAEGAEN

      • 预测分子量

        31 kDa including tags

      • 氨基酸

        1 to 245

      • 标签

        His tag N-Terminus

    技术指标

    Our Abpromise guarantee covers the use of ab116444 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Mass Spectrometry

      SDS-PAGE

    • 质谱法

      MALDI-TOF-TOF

    • 形式

      Liquid

    • 补充说明

      ab116444 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF mass spectrometry. The peptides obtained gave 64 % sequence coverage of 14-3-3 tau + theta. Theoretical pI: 5.2

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.012% Benzamidine, 0.004% PMSF, 0.02% DTT, 0.6% HEPES, 50% Glycerol

    常规信息

    • 别名

      • 14-3-3 protein T cell
      • 14-3-3 protein T-cell
      • 14-3-3 protein tau
      • 14-3-3 protein theta
      • 1433T_HUMAN
      • IC5
      • Protein HS1
      • Protein tau
      • Ywhaq
      • YWHAQ tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
      see all

    • 功能

      Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

    • 组织特异性

      Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.

    • 序列相似性

      Belongs to the 14-3-3 family.

    • 翻译后修饰

      Ser-232 is probably phosphorylated by CK1.

    • 细胞定位

      Cytoplasm. In neurons, axonally transported to the nerve terminals.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human 14-3-3 Theta + Tau protein (ab116444)
      SDS-PAGE - Recombinant Human 14-3-3 Theta + Tau protein (ab116444)
      12% SDS-PAGE gel showing ab116444.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab116444？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab116444 尚未被引用在任何文献中。

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