Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

重组人14-3-3 gamma/YWHAG蛋白(ab53869)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (ab53869)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Suitable for: SDS-PAGE

    描述

    • 产品名称

      重组人14-3-3 gamma/YWHAG蛋白
      参阅全部 14-3-3 gamma/YWHAG 蛋白酶

    • 纯度

      > 95 % SDS-PAGE.
      Recombinant human 14-3-3 was expressed in E.coli and purified by using conventional chromatography techniques.

    • 内毒素水平

      < 1.000 Eu/µg

    • 表达系统

      Escherichia coli

    • Accession

      P61981

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN

      • 预测分子量

        28 kDa

      • 氨基酸

        1 to 247

    技术指标

    Our Abpromise guarantee covers the use of ab53869 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      SDS-PAGE

    • 形式

      Liquid

    • 补充说明

      This product was previously labelled as 14-3-3 gamma.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 7.40
      Constituents: PBS, 10% Glycerol (glycerin, glycerine)

    常规信息

    • 别名

      • 14 3 3 gamma
      • 14 3 3 protein gamma
      • 14 3 3 protein gamma subtype
      • 14 3 3gamma
      • 14-3-3 protein gamma
      • 1433G_HUMAN
      • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide
      • KCIP 1
      • KCIP-1
      • KCIP1
      • N-terminally processed
      • Protein kinase C inhibitor protein 1
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide
      • Ywhag
      see all

    • 功能

      Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

    • 组织特异性

      Highly expressed in brain, skeletal muscle, and heart.

    • 序列相似性

      Belongs to the 14-3-3 family.

    • 翻译后修饰

      Phosphorylated by various PKC isozymes.

    • 细胞定位

      Cytoplasm.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (ab53869)
      SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (ab53869)
      ab53869 on 15% SDS-PAGE

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab53869？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab53869 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    What kind of chromatography is used to purify the protein? If the protein is tagged, what kind of tag is it and where is the tag?

    Read More
    Answer

    Thank you for your call yesterday. I have heard back from the lab, and the protein ab53869 is not tagged. The protein is purified via a proprietary chromatography technique that does not require a tag. I hope this information is helpful, but please let me know if there is anything else that we can do for you.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com