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重组HSV2 gG Envelope蛋白(ab43047)

Submit a review Q&A (2)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: GST tag N-Terminus
  • Suitable for: ELISA, WB

描述

  • 产品名称

    重组HSV2 gG Envelope蛋白

  • 生物活性

    Immunoreactive with sera of HSV-infected individuals, excellent antigen for detection of HSV with minimal specificity problems.

  • 纯度

    > 95 % SDS-PAGE.
    Sepharose derived purification.

  • 表达系统

    Escherichia coli

  • 蛋白长度

    Protein fragment

  • 无动物成分

    No

  • 性质

    Recombinant

    • 实际分子量

      31 kDa

    • 氨基酸

      525 to 578

    • 标签

      GST tag N-Terminus

    • 额外的序列信息

      The protein contains the HSV-2 gG immunodominant regions. GST tagged.

技术指标

Our Abpromise guarantee covers the use of ab43047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    Western blot

  • 形式

    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C.

    Constituents: 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol

常规信息

  • 别名

    • Envelope glycoprotein G
    • GG
    • Glycoprotein G
    • Herpes simplex virus 2 glycoprotein G
    • HHV2 gG envelope protein
    • US4
    see all

  • 相关性

    HSV2 is a member of the herpes virus family, Herpesviridae. All herpes viruses are morphologically identical. They have a large double stranded DNA genome. The virion consists of an icosahedral nucleocapsid which is surrounded by a lipid bilayer envelope. Between the capsid and the envelope is an amorphous layer of proteins, termed the tegument. Glycoprotein G (gG) is one of seven envelope glycoproteins in HSV2.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab43047？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab43047 尚未被引用在任何文献中。

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

I’m trying to locate HSV monoclonal antibodies for use in western immunoblots. Specifically I need HSV-1 gC-1 (130kDa), HSV-2 gG-2 (92kDa) and HSV-2 140kDa glycoprotein. Your ABCAM on-line catalogue lists envelope proteins for both HSV-1 and -2 but does not seem to list their molecular weights. Is it possible to try some samples if you can get these monoclonals?

Could you please try monoclonals to HSV1 gG ab67739, HSV2 gG ab43047 and HSV2 gG ab67703. There does not seem to be any HSV1 gC. Could you ask if they can get some?

Could you please advise the molecular weight for the antibodies above, and if there is any plan for an HSV1 gc antibody to be produced?

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Answer

Thank you for contacting us.

The reference numbers ab67739, ab43047 and ab67703 given by your customer are not monoclonal antibodies but are the proteins. Can you check with them that this is indeed what they want?

Please let your customer know that we are not specialized in this particular field but I am happy to recommend antibodies.

We have ab6511 which is an anti-HSV1 gG Envelope Protein antibody [7F5] (https://www.abcam.com/6511).

We have several anti-HSV2 gG antibodies which I could also suggest.

Regarding an anti-HSV1 gC, we do have an Anti-HSV1 gC Envelope Protein antibody [3G9], ab6509 (https://www.abcam.com/ab6509) which detects a band at 95 kDa.

As for the molecular weights, they are not on the datasheets and I do not have images to refer to for all of these antibodies. Could you ask your customer where he got his predicted molecular weights from because I have had a thorough look and can only find lower molecular weight around 50 kDa for these proteins?

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Thanks for the response.
Would you be able to send me a cost for the material?
Thanks,

Read More
Answer

Thank you for getting back to me. The standard cost of these materials is as follows:
HSV1 proteins:
Ab124003 (clarified crude cell lysate) £283.00, 1 mL
Ab124004 (concentrated and purified cell lysate) £335.00, 200 uL
Ab43048 (recombinantly expressed) £175.00, 200 ug (at 1 mg/mL)
HSV2 proteins:
Ab124005 (clarified crude cell lysate) £283.00, 1 mL
Ab124006 (concentrated and purified cell lysate) £335.00, 200 uL
Ab43047 (recombinantly expressed) £175.00, 200 µg (at 1 mg/mL)
However, if you would be able to let me know which product you are interested in and how much you are likely to want I can pass this on to my colleague to determine if we can provide this to you at a discount.
I hope this information has been of help. If you have any further questions please do not hesitate to contact me again.

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