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重组HPV18 L1蛋白(ab119881)

Submit a review Q&A (5)References (1)
SDS-PAGE - Recombinant HPV18 L1 protein (ab119881)
  • Western blot - Recombinant HPV18 L1 protein (ab119881)
  • Electron Microscopy - Recombinant HPV18 L1 protein (ab119881)
  • SDS-PAGE - Recombinant HPV18 L1 protein (ab119881)

Key features and details

  • Expression system: Saccharomyces cerevisiae
  • Purity: > 90% SDS-PAGE
  • Suitable for: WB, SDS-PAGE, Electron Microscopy

描述

  • 产品名称

    重组HPV18 L1蛋白

  • 纯度

    > 90 % SDS-PAGE.
    ab119881 is purified by ultracentifugation.

  • 表达系统

    Saccharomyces cerevisiae

  • Accession

    AAQ92369.1

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 序列

      MALWRPSDNTVYLPPPSVARVVNTDDYVTRTSIFYHAGSSRLLTVGNPYF RVPAGGGNKQDIPKVSAYQYRVFRVQLPDPNKFGLPDTSIYNPETQRLVW ACAGVEIGRGQPLGVGLSGHPFYNKLDDTESSHAATSNVSEDVRDNVSVD YKQTQLCILGCAPAIGEHWAKGTACKSRPLSQGDCPPLELKNTVLEDGDM VDTGYGAMDFSTLQDTKCEVPLDICQSICKYPDYLQMSADPYGDSMFFCL RREQLFARHFWNRAGTMGDTVPQSLYIKGTGMRASPGSCVYSPSPSGSIV TSDSQLFNKPYWLHKAQGHNNGVCWHNQLFVTVVDTTRSTNLTICASTQS PVPGQYDATKFKQYSRHVEEYDLQFIFQLCTITLTADVMSYIHSMNSSIL EDWNFGVPPPPTTSLVDTYRFVQSVAITCQKDAAPAENKDPYDKLKFWNV DLKEKFSLDLDQYPLGRKFLVQAGLRRKPTIGPRKRSAPSATTSSKPAKR VRVRARK

    • 预测分子量

      57 kDa

    • 氨基酸

      1 to 507

技术指标

Our Abpromise guarantee covers the use of ab119881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

    Electron Microscopy

  • 形式

    Lyophilized
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Constituent: 99% PBS

  • 复溶
    Reconstitute in 50 µl of water. Then dilute with PBS to the required concentration.

常规信息

  • 别名

    • L1
    • HPV18 major capsid protein L1
    • Human papilloma virus type 18 major capsid protein L1
    • Human papillomavirus type 18 L1
    • Human papillomavirus type 18 major capsid protein L1
    • Major capsid protein L1
    see all

  • 相关性

    L1 is a major capsid protein of type 18 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.

  • 细胞定位

    Virion

图片

  • SDS-PAGE - Recombinant HPV18 L1 protein (ab119881)
    SDS-PAGE - Recombinant HPV18 L1 protein (ab119881)

    SDS-PAGE analysis of ab119881.

    Lane 1. ab119881
    Lane 2. MWt marker

  • Western blot - Recombinant HPV18 L1 protein (ab119881)
    Western blot - Recombinant HPV18 L1 protein (ab119881)
    Recombinant HPV18 L1 protein (ab119881)

    Predicted band size: 56.5 kDa



    Western blot analysis of ab119881.

    Lane 1. ab119881
    Lane 2. MWt marker

  • Electron Microscopy - Recombinant HPV18 L1 protein (ab119881)
    Electron Microscopy - Recombinant HPV18 L1 protein (ab119881)
    ab119881 viewed by electron microscopy.
  • SDS-PAGE - Recombinant HPV18 L1 protein (ab119881)
    SDS-PAGE - Recombinant HPV18 L1 protein (ab119881)
    SDS-PAGE analysis of ab119881.

    Lane 1. ab119881, 3 µg/lane
    Lane 2. MWt marker 11.0, 17.0, 26.0, 34.0, 43.0, 55.0, 72.0, 95.0, 130.0, 170.0 kDa.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab119881？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab119881 被引用在 1 文献中.

  • Pedroza-Gonzalez A  et al. Human papillomavirus infection and seroprevalence among female university students in Mexico. Hum Vaccin Immunother 18:2028514 (2022). PubMed: 35103580

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Question

Hope this e-mail finds you well!
Please note that one of our customer recently purchased the antibodies viz. ab119881& ab31492 & is asking for below informations:


1 .Leaf let its mentioned reconstitute in PBS of HPV18L1 protein (ab119881), What concentration PBS is required?

2. HPV18L1 protein is mentioned molecularweight is 56.5kDa Please send me sample preparation of this protein for SDSPAGE method?

3.This is Virus like particle(VLP ) protein directly i could not get any band by SDSPAGE method .



Please send me sample preparation protocol for SDS-PAGE method . Weneed these details for optimization purpose.



Kindly advise us on the same.


Looking forward towards your valued response on it.

With Best Regards,

Read More
Answer

Thank you for contacting us.

The protein can be reconstituted in 1X PBS.

No special protocol is needed for SDS-PAGE any general SDS-PAGE protocol will work. Please mix the protein with 1:1 loading buffer and boil for 5-10 minutes before loading into gel.

Try loading 5-10ug of protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

What is the amino acid sequence of the HPV18 L1 protein?

Read More
Answer

The sequence is the 507aa GenBank gb|AAQ92369 sequence.

http://www.ncbi.nlm.nih.gov/protein/AAQ92369.1

Read More

Question

Thank you for your reply.

Customer received four vials of ab119881 & did not get the protein inside one vial after doing recommended reconstitution also.

Kindly arrange for further needful on it, since this is a very high potential regular user of our Abcam products.

Looking forward towards your valued reply on it.

With Best Regards,

Read More
Answer

Thank you for providing that information.

I am sorry for the misunderstanding in this case. I am sorry to hear that the customer has not received the expected products in one of the vials. I would be happy to send a replacement to you if you would be able to provide me with the order number on which the 4 vials of ab119881 was ordered.

I have found4 separateorders of the following dates from you:



Are these the orders concerned?

Do you know which order the problem vial corresponds to?

If I arrange for a replacement vial of ab119881 to be sent could you please confirm the address I have for you is correct:
xxxxxxxxxxxxxxxxxxxx




Many thanks for your cooperation. I look forward to receiving your reply.

Read More

Question

Dear Sir,

Today we have received HPV18L1 lyophilized protein(ab119881)& Anti-HPV18L1 antibody(ab31492) from Abcam

I need to know,

1 .Leaf let its mentioned reconstitute in PBS of HPV18L1 protein What concentration PBS is required?

2. HPV18L1 protein is mentioned molecularweight is 56.5kDa Please send me sample preparation of this protein for SDSPAGE method?

3.This is Virus like particle(VLP ) protein directly i could not get any band by SDSPAGE method

Please send me sample preparation protocol for SDS-PAGE method . We have many VLP like 16,6,11 protein projects .

Once we will optimize this protein preparation feature we will purchse more antigens& Antibodys from Abcam

Awating for your reply.


With Regards,

Read More
Answer

Thank you for your enquiry and your interest in our products.

Please find the information regarding ab119881 below:

Question 1.)

If you dissolve the HPV18L1 protein (50 micrograms) in 50 microliters of distilled water (the storage buffer is PBS) the concentration of HPV18L1 protein will be 1 mg/ml. If you need more diluted protein, please dilute it with PBS further.

Question 2.)

The theoretical molecular weight of HPV18L1 protein is 56.5kDa but the actual weight could be slightly different. For loading on SDS PAGE (minigel) you need to prepare the dissolved HPV18L1 protein (1mg/ml) as follows:

Prepare 90 microliters of Protein Sample or Loading Buffer x1 (you can prepare it yourself or purchase it ready-made) and add 10 microliters of the dissolved HPV18L1 protein (1mg/ml). Boil the mix 10 min and load on the gel 10 microliters per track of the cooled mix.

Question 3.)

If you prepare the HPV18L1 protein as explained above you will see 1 microgram per band on SDS PAGE after staining of the gel with Coomassie Blue.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Question

Dear Technical Team,

Hope this e-mail finds you well!

Kindly look into the belowmail from one this customer & advise us on it, enabling us to update him accordingly.

Looking forward towards your valued reply/ comments on it.

Thankingyou in advance.

With Best Regards,

I spoke to you regarding HPV18L1 antigen we had received 4 vials
1 vial has not having antigen . Please find the following antigen details.

ab119881

lot number is GR72034-3

Kindly replace the material as soon as possible .

Regards,

Read More
Answer

Thank you for contacting us.

I am sorry but I am a little unsure of what the customer has asked for. Has he found no product in the vial of ab119881 received?

The HPV18 L1 protein (ab119881) is the recombinantly expressed, full length HPV18 L1 protein which has been lypholised. The protein needs to be reconsituted prior to use, it is provided as a solid and may not be visible in the vial itself. But please be assured that 50 ug of protein is provided in each vial which can be reconsituted to the concentration of your choice using PBS.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

Read More

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