Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: ELISA, WB
参阅全部 HIV1 Nef 蛋白酶
生物活性Reacts strongly with human HIV positive serum.
纯度> 95 % SDS-PAGE.
额外的序列信息Fused with β-galactosidase at the N-terminus.
Our Abpromise guarantee covers the use of ab49060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
补充说明Reacts strongly with human HIV positive serum.
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Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 0.106% Sodium carbonate, 0.1092% Beta mercaptoethanol, 0.292% EDTA
- C terminal core protein
- F protein
相关性Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.