重组HIV1 gp41蛋白(ab49070)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: ELISA, WB
描述
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产品名称
重组HIV1 gp41蛋白
参阅全部 HIV1 gp41 蛋白酶 -
纯度
> 90 % SDS-PAGE.
Purified by affinity chromatography. -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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序列
IEFPGIFRPG GGDMRDNWRS ELYKYKVVKI EPLGVAPTKA KRRVVQREKR AVGIGALFLG FLGAAGSTMG AASMTLTVQA RQLLSGIVQQ QNNLLRAIEA QQHLLQLTVW GIKQLQARIL AVERYLKDQQ LLGIWGCSGK LICTTAVPWN ASWSNKSLEQ IWNNMTWMEW DREINNYTSL IHSLIEESQN QQEKNEQEL LELDKWASLW NWFNITNWLW YIKLFIMIVG GLVGLRIVFA VLSVVNRVRQ GYSPLSFQTH LPIPRGPDRP EGIEEEGGER DRDRSIRLVN GSLALIWDDL RSLCLFSYHR LRDLLLIVTR IVELLGRRGW EALKYWWNLL QYWSQELKNS AVSLLNATAI AVAEGTDRVI EVVQGAYRAI RHIPRRIRQG LERILL -
额外的序列信息
Fusions β-galactosidase (114 kDa) fused at the N-terminus
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技术指标
Our Abpromise guarantee covers the use of ab49070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
ELISA
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% Urea, 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol (glycerin, glycerine)
常规信息
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别名
- Env
- Glycoprotein 41
- gp41
see all -
相关性
gp41/120 is the major HIV protein associated with the HIV envelope. It functions as the viral antireceptor or attachment protein. gp41 (or TM) traverses the envelope, whereas gp120 is present on the outer surface and is noncovalently attached to gp41. The precursor of gp120/41 (gp160) is synthesized in the endoplasmic reticulum and is transported via the golgi body to the cell surface. Upon activation of the envelope glycoprotein (gp120/41) by cellular receptors, gp41 undergoes conformational changes that mediate fusion of the viral and cellular membranes.
图片
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ELISA using ab49070 at 2µg/mL Antibodies which recognise the 2F5 and 4E10 epitope were used as primary antibodies, and Protein A HRP was used as a seconday antibody.
Significance: gp41-specific antibodies which bind two different epitopes are able to recognize this full length gp41 in ELISA
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab49070 被引用在 2 文献中.
- Caires AJ et al. Gold nanoparticle-carboxymethyl cellulose nanocolloids for detection of human immunodeficiency virus type-1 (HIV-1) using laser light scattering immunoassay. Colloids Surf B Biointerfaces 177:377-388 (2019). PubMed: 30785035
- Miles P et al. In vitro assembly of a viral envelope. Soft Matter 11:7722-7 (2015). PubMed: 26299568