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重组丙型肝炎病毒genotype 1a NS5蛋白(ab49033)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Suitable for: ELISA, WB, Flow Cyt

描述

  • 产品名称

    重组丙型肝炎病毒genotype 1a NS5蛋白

  • 纯度

    > 95 % SDS-PAGE.

  • 表达系统

    Escherichia coli

  • 蛋白长度

    Protein fragment

  • 无动物成分

    No

  • 性质

    Recombinant

    • 氨基酸

      2212 to 2313

技术指标

Our Abpromise guarantee covers the use of ab49033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    Western blot

    Flow Cytometry

  • 形式

    Liquid

  • 补充说明

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 7.2
    Constituents: 0.2% Triton-X-100, 9% Urea, 0.395% Tris HCl, 50% Glycerol (glycerin, glycerine)

常规信息

  • 别名

    • HCV 1a NS5
    • Hepatitis C virus NS5A
    • Non-structural protein 5A
    • p56

  • 相关性

    Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008). NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication. Hepatitis C virus (HCV) can cause chronic hepatitis, cirrhosis and hepatocellular carcinoma. At present there is no vaccine effective against HCV.

  • 细胞定位

    Endoplasmic reticulum membrane

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab49033？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab49033 尚未被引用在任何文献中。

客户评价及客户问答

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Question


I’ve attached the protocol I used for the Western blot. I’m trying to find a good antibody to HCV NS5A so that I can select stable clones overexpressing the protein. So far, I haven’t been able to pick up any NS5A using two proteins from Abcam, ab49033 and ab68096, and high concentrations (1:100, 1:250) of four different antibodies (ab20342, sc-57776, ab20773, ab2594).

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Answer

Thanks for sending along your protocol.

1. ab49033 is unlikely to be detected by these antibodies as it only contains about 100 aa of the total protein. As described on datasheet this product containsamino acids"corresponding to amino acids 2212-2313 of Hepatitis C Virus NS5" The epitopes recognized by these antibodies would have to recognize NS5A within this small region to work.

2. Usingponceau S stain post transfer, have you been able to determine protein is present on membrane after transfer?

3. Regarding your protocol, a) Boiling of samplesis not recommended; instead heat at 70 deg C for 10min prior to loading gel b) Try blocking with 5% BSA instead of milk c) Use 1:1000 for primary antibodies and 1:5000 for secondary antibodies.

I look forward to hearing if these suggestions prove useful.

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