重组Enhanced GFP蛋白(His tag) (ab134853)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: = 5.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组Enhanced GFP蛋白(His tag)
参阅全部 GFP 蛋白酶 -
纯度
> 95 % SDS-PAGE.
ab134853 was expressed in E.coli as soluble protein and was purified using a Ni-NTA column. -
内毒素水平
= 5.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Aequorea victoria -
序列
MGDIMGEWGNEIFGAIAGFLGVSKGEELFTGVVPILVELDGDVNGHKFSV SGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYV QERTIFFKDDGNYK TRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ KNGIKVNFKIRHNI EDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDE LYKSRHRRHRQRSRSRAAARRRRRRRRRHHHHHH -
预测分子量
33 kDa -
氨基酸
2 to 238 -
标签
His tag C-Terminus -
额外的序列信息
Second generation monomeric GFP (Enhanced GFP). Constructed with a N-terminal tag of HA2 peptide and C-terminal 9 arginine domain/His Tag.
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描述
重组 Enhanced GFP蛋白(His tag)
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab134853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
补充说明
This version of GFP is the second generation monomeric green fluorescent protein (Enhanced GFP) that has improved brightness and photostability.
This EGFP-9R, have a single excitation peak centered at about 488 nm, with an emission peak wavelength of 509 nm.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
常规信息
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别名
- GFP
- Green fluorescent protein
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相关性
Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab134853 被引用在 5 文献中.
- Mann FA et al. Quantum defects as versatile anchors for carbon nanotube functionalization. Nat Protoc 17:727-747 (2022). PubMed: 35110739
- Hunter P et al. Single-molecule and super-resolved imaging deciphers membrane behavior of onco-immunogenic CCR5. iScience 25:105675 (2022). PubMed: 36561885
- Elguindy MM & Mendell JT NORAD-induced Pumilio phase separation is required for genome stability. Nature 595:303-308 (2021). PubMed: 34108682
- Castro L et al. Kir6.2, the Pore-Forming Subunit of ATP-Sensitive K+ Channels, Is Overexpressed in Human Posttraumatic Brain Contusions. J Neurotrauma 36:165-175 (2019). PubMed: 29737232
- Scherr TF et al. A two-magnet strategy for improved mixing and capture from biofluids. Biomicrofluidics 10:024118 (2016). PubMed: 27158286