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AB72847

重组 E. coli slyD 蛋白 (Tag Free)

Recombinant E. coli slyD protein (Tag Free)

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Recombinant E. coli slyD protein (Tag Free) is a Escherichia coli CFT073 Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

查看别名

c4123, slyD, FKBP-type peptidyl-prolyl cis-trans isomerase SlyD, PPIase, Metallochaperone SlyD

1 Images
SDS-PAGE - Recombinant E. coli slyD protein (Tag Free) (AB72847)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant E. coli slyD protein (Tag Free) (AB72847)

SDS-PAGE showing ab72847 (3μg) at approximately 21kDa.

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

Tag free

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH 8.0 using chymotrypsin.

访问

P0A9L0

不含动物源

No

不含载体蛋白

No

种属

Escherichia coli CFT073

存储溶液

pH: 7.5 Constituents: 0.242% Tris

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHHDHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0A9L0","tags":[]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SlyD also known as FKBP-like peptidyl prolyl cis-trans isomerase weighs approximately 23 kDa. It functions mechanically as a protein-folding chaperone and possesses peptidyl-prolyl isomerase (PPIase) activity helping proteins achieve their correct conformations. SlyD is expressed in the cytoplasm where it interacts with various cellular components due to its ability to bind metal ions and other proteins.
Biological function summary

The SlyD protein serves important roles in cellular homeostasis by assisting in protein folding and stabilization of cellular stress responses. It acts independently as well as a component of larger protein complexes. Its activity aids in maintaining the functionality of unfolded or misfolded proteins that might otherwise aggregate which is important for preventing cellular stress and potential damage.

Pathways

SlyD plays an important role in the protein homeostasis and stress response pathways. It engages in cellular processes related to the cellular stress response especially as it connects with heat shock proteins like Hsp70. SlyD also associates with proteins part of the folding pathway supporting both protein synthesis under normal conditions and recovery during stress.

Research has shown SlyD's involvement in neurodegenerative diseases and bacterial infections. Aberrant protein folding processes linked to SlyD have implications for neurodegenerative diseases like Alzheimer's where protein aggregation is a factor. During bacterial infections SlyD's interaction with host proteins can influence pathogenicity making it a potential target for therapeutic interventions.
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特殊说明

形式

Liquid

附加说明

ab72847 is purified by conventional chromatography.

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常规信息

功能

Folding helper with both chaperone and peptidyl-prolyl cis-trans isomerase (PPIase) activities. Chaperone activity prevents aggregation of unfolded or partially folded proteins and promotes their correct folding. PPIases catalyze the cis-trans isomerization of Xaa-Pro bonds of peptides, which accelerates slow steps of protein folding and thus shortens the lifetime of intermediates. Both strategies lower the concentration of intermediates and increase the productivity and yield of the folding reaction. SlyD could be involved in Tat-dependent translocation, by binding to the Tat-type signal of folded proteins (By similarity).. Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity (By similarity).

序列相似性

Belongs to the FKBP-type PPIase family.

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产品实验方案

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靶点信息

Folding helper with both chaperone and peptidyl-prolyl cis-trans isomerase (PPIase) activities. Chaperone activity prevents aggregation of unfolded or partially folded proteins and promotes their correct folding. PPIases catalyze the cis-trans isomerization of Xaa-Pro bonds of peptides, which accelerates slow steps of protein folding and thus shortens the lifetime of intermediates. Both strategies lower the concentration of intermediates and increase the productivity and yield of the folding reaction. SlyD could be involved in Tat-dependent translocation, by binding to the Tat-type signal of folded proteins (By similarity).. Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity (By similarity).
See full target information slyD
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