重组E. coli RuvC蛋白(Active) (ab63828)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, ELISA, Functional Studies
描述
-
产品名称
重组E. coli RuvC蛋白(Active) -
纯度
> 90 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Escherichia coli -
序列
MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDD LPSRLKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIV AAVNQELPVFEYAARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADA ADALAIAITHCHVSQNAMQMSESRLNLARGRLR
-
-
描述
重组E. coli RuvC蛋白(Active)
技术指标
Our Abpromise guarantee covers the use of ab63828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
Western blot
ELISA
Functional Studies
-
形式
Liquid -
补充说明
ab63828 can be used for: 1) Studies on the homologous recombination mechanism. 2) To use as an endonuclease which functions specifically to the Holliday structure.
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Store at -20°C or -80°C. Stable for 12 months at -20°C.
pH: 6
Constituents: 0.039% Beta mercaptoethanol, 0.158% Tris HCl, 0.0584% EDTA, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- Crossover junction endodeoxyribonuclease ruvC
- Holliday junction nuclease ruvC
- Holliday junction resolvase ruvC
-
相关性
In Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double stranded DNA and enhances ATPase activity of RuvB. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC, RuvAB is uncoupled from RuvC and DSBs may be prevented.
图片
-
SDS-PAGE - Recombinant E. coli RuvC protein (Active) (ab63828)
SDS-PAGE analysis of Recombinant E. coli RuvC protein (ab63828).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab63828 被引用在 3 文献中.
- Ray S et al. Single bacterial resolvases first exploit, then constrain intrinsic dynamics of the Holliday junction to direct recombination. Nucleic Acids Res 49:2803-2815 (2021). PubMed: 33619520
- Nicholls TJ et al. Topoisomerase 3a Is Required for Decatenation and Segregation of Human mtDNA. Mol Cell 69:9-23.e6 (2018). PubMed: 29290614
- García-Luis J & Machín F Mus81-Mms4 and Yen1 resolve a novel anaphase bridge formed by noncanonical Holliday junctions. Nat Commun 5:5652 (2014). PubMed: 25466415