重组E. coli RecQ DNA Helicase蛋白(Active) (ab63813)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组E. coli RecQ DNA Helicase蛋白(Active) -
生物活性
Unwinding duplex DNA, dependent on ATP. DNA-dependent ATPase.
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纯度
> 90 % SDS-PAGE.
Highly purified by several steps of chromatography. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Escherichia coli -
序列
MAQAEVLNLE SGAKQVLQET FGYQQFRPGQ EEIIDTVLSG RDCLVVMPTG GGKSLCYQIP ALLLNGLTVV VSPLISLMKD QVDQLQANGV AAACLNSTQT REQQLEVMTG CRTGQIRLLY IAPERLMLDN FLEHLAHWNP VLLAVDEAHC ISQWGHDFRP EYAALGQLRQ RFPTLPFMAL TATADDTTRQ DIVRLLGLND PLIQISSFDR PNIRYMLMEK FKPLDQLMRY VQEQRGKSGI IYCNSRAKVE DTAARLQSKG ISAAAYHAGL ENNVRADVQE KFQRDDLQIV VATVAFGMGI NKPNVRFVVH FDIPRNIESY YQETGRAGRD GLPAEAMLFY DPADMAWLRR CLEEKPQGQL QDIERHKLNA MGAFAEAQTC RRLVLLNYFG EGRQEPCGNC DICLDPPKQY DGSTDAQIAL STIGRVNQRF GMGYVVEVIR GANNQRIRDY GHDKLKVYGM GRDKSHEHWV SVIRQLIHLG LVTQNIAQHS ALQLTEAARP VLRGESSLQL AVPRIVALKP KAMQKSFGGN YDRKLFAKLR KLRKSIADES NVPPYVVFND ATLIEMAEQM PITASEMLSV NGVGMRKLER FGKPFMALIR AHVDGDDEE -
预测分子量
68 kDa -
氨基酸
1 to 609
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描述
重组E. coli RecQ DNA Helicase蛋白(Active)
技术指标
Our Abpromise guarantee covers the use of ab63813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
Applications
1) DNA dependent ATPase.
2) DNA helicase specific for various form of DNA structure like branched form and fork.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 6.00
Constituents: 0.38% Potassium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.316% Tris HCl, 0.0292% EDTA, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- ATP dependent DNA helicase
- ATP dependent DNA helicase recQ
- DNA helicase
see all -
相关性
RecQ is a DNA helicase, involved in the recF recombination pathway; its gene expression is under the regulation of the SOS system. -
细胞定位
Cytoplasmic, Bacterial nucleoid, Replisome
图片
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SDS-PAGE - Recombinant E. coli RecQ DNA Helicase protein (ab63813)
SDS-PAGE analysis of Recombinant E. coli RecQ DNA Helicase protein (ab63813).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab63813 被引用在 3 文献中.
- Khan I et al. Impact of age-associated cyclopurine lesions on DNA repair helicases. PLoS One 9:e113293 (2014). PubMed: 25409515
- Suhasini AN et al. DNA repair and replication fork helicases are differentially affected by alkyl phosphotriester lesion. J Biol Chem 287:19188-98 (2012). PubMed: 22500020
- Hishida T et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev 18:1886-97 (2004). PubMed: 15289460