重组E. coli RecA蛋白(Active) (ab174091)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE, Electron Microscopy
描述
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产品名称
重组E. coli RecA蛋白(Active)
参阅全部 RecA 蛋白酶 -
纯度
> 90 % SDS-PAGE.
ab174091 was over-expressed as a recombinant protein and highly purified by several steps of chromatography. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Escherichia coli -
序列
MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDI ALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHAL DPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAAL TPKAEIEGEIGDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKI GVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKN KIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIG QGKANATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSVDDSEGVAETN EDF -
预测分子量
36 kDa -
氨基酸
1 to 353
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描述
重组E. coli RecA蛋白(Active)
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab174091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Electron Microscopy
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 0.32% Tris-HCl buffer, 0.03% EDTA, 1.1% Potassium chloride, 0.02% DTT
常规信息
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别名
- DNA strand exchange and recombination protein with protease and nuclease activity
- lexB
- Protein recA
see all -
相关性
RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. -
细胞定位
Cytoplasmic
图片
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SDS-PAGE - Recombinant E. coli RecA protein (Active) (ab174091)
SDS-PAGE analysis of purified Recombinant E. coli RecA protein (ab174091).
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SDS-PAGE - Recombinant E. coli RecA protein (Active) (ab174091)Polyacrylamide gel electrophoresis of ab174091.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab174091 被引用在 1 文献中.
- Horii T et al. Regulation of SOS functions: purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein. Cell 27:515-22 (1981). Functional Studies ; Escherichia coli . PubMed: 6101204