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重组E. coli Multi Tag蛋白(ab36791)

Submit a review Q&A (2)References (1)
Western blot - Recombinant <em>E. coli </em> Multi Tag protein (ab36791)

    Key features and details

    • Expression system: Escherichia coli
    • Suitable for: WB

    描述

    • 产品名称

      重组E. coli Multi Tag蛋白

    • 生物活性

      This protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis.

    • 表达系统

      Escherichia coli

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Escherichia coli

      • 预测分子量

        45 kDa

    技术指标

    Our Abpromise guarantee covers the use of ab36791 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Western blot

    • 形式

      Liquid

    • 补充说明

      This protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis.

      This product is a bacterial lysate provided in1 x reduced SDS-PAGE loading buffer. It is effective as a positive control for epitope tag antibodies in immunoblotting. 

      For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µl. It is recommended that the reagent be titrated for optimal performance for each application.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

      pH: 6.80
      Constituents: 0.76% Tris, 2% Sodium lauryl sulfate, 10% Glycerol, 0.01% Bromophenol blue

    图片

    • Western blot - Recombinant <em>E. coli </em> Multi Tag protein (ab36791)
      Western blot - Recombinant E. coli Multi Tag protein (ab36791)
      All lanes : Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µl

      Lane 1 : HA-HRP
      Lane 2 : Myc-HRP
      Lane 3 : Glu-Glu
      Lane 4 : GST
      Lane 5 : His
      Lane 6 : HSV
      Lane 7 : T7
      Lane 8 : V5
      Lane 9 : VSV
      Lane 10 : S-Tag
      Lane 11 : FLAG-HRP

      Predicted band size: 45 kDa

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab36791？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab36791 被引用在 1 文献中.

    • Trofka A  et al. The Her7 node modulates the network topology of the zebrafish segmentation clock via sequestration of the Hes6 hub. Development 139:940-7 (2012). WB . PubMed: 22278920

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    1-2 of 2 Abreviews or Q&A

    Question

    I am planning to quantify a myc-tagged protein in a lysate by Western Blot. I am thus wondering if product ab36791 (multi-tag refernece protein) is a purified recombinant protein that comes at a specific (lot-specific) protein concentration or is it just a bacterial lysate?

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    Answer

    I confirm that the multi-tag protein ab36791 is prepared from the whole bacteria lysate, therefore the concentration cannot be determined.
    The recommended usage of this product is between 0.5 and 2 µl per well for a standard western blot procedure.

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    Question

    ab36791 Multi Tag protein Can you clarify the following: 1) Are the different tags sequentially tagged to a protein or? 2) Is there a spacer sequence between each tag? 3) Are the tags just tagged to one protein or to different ones? 4) What protein(s) is being tagged? 5) Do you have a WB image of this product with use of the different tag-antibodies?

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    Answer

    Thank you for your patience in this matter. The originator of ab36791 has informed me that the tags are sequential and that they are attached to a 41kDa recombinant protein. We will soon add a WB image to the product datasheet. I hope this information helps.

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