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重组E. coli Beta-lactamase蛋白(ab104926)

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SDS-PAGE - Recombinant <em>E. coli </em> Beta-lactamase protein (ab104926)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    描述

    • 产品名称

      重组E. coli Beta-lactamase蛋白
      参阅全部 Beta-lactamase 蛋白酶

    • 纯度

      > 95 % SDS-PAGE.
      ab104926 was purified using conventional chromatography.

    • 表达系统

      Escherichia coli

    • Accession

      P00811

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Escherichia coli

      • 序列

        MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ

      • 预测分子量

        42 kDa including tags

      • 氨基酸

        20 to 377

      • 标签

        His tag N-Terminus

    • 描述

      重组E. coli Beta-lactamase蛋白

    技术指标

    Our Abpromise guarantee covers the use of ab104926 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      SDS-PAGE

      Mass Spectrometry

    • 质谱法

      MALDI-TOF

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

    常规信息

    • 别名

      • ampA
      • ampC
      • Cephalosporinase

    • 相关性

      This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

    图片

    • SDS-PAGE - Recombinant <em>E. coli </em> Beta-lactamase protein (ab104926)
      SDS-PAGE - Recombinant E. coli Beta-lactamase protein (ab104926)
      15% SDS-PAGE analysis of 3ug ab104926

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab104926？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab104926 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    I am interested in the following two products:

    1) Ab against Beta-Lactamase (ab12251) and purified Beta-Lactamase protein (ab104926)

    Can you confirm that the above mentioned antibody (ab12251) recognized the purified B-Lactamase protein (ab104926). If so, then I would need a formal offer (preferentially in PDF format) to place the order.

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    Answer

    Thank you for contacting us.

    ab12251's immunogen is 5'-His-tagged E.coli TEM-1 beta lactamase therefore this antibody is specific to TEM-1 beta lactamases. I have compared the sequence similarity between TEM-1 beta lactamase and ab104926 and only found 10% sequence similarity so it is unlikely that this antibody will detect this protein.

    However, I have found another protein, beta lactamase TEM precursor protein, ab67672 (https://www.abcam.com/ab67672) which has a 99.6% sequence similarity with TEM-1 beta lactamase. Therefore, this protein is likely to be detected by ab12251.

    If you would like a proforma for these two products, please let me know and I will be more than happy to do so.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
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