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AB268312

重组E. coli Acetate Kinase蛋白(Tagged)

Recombinant E. coli Acetate Kinase protein (Tagged)

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Recombinant E. coli Acetate Kinase protein (Tagged) is a Escherichia coli K-12 Full Length protein, in the 1 to 400 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

ack, b2296, JW2293, ackA, Acetate kinase, Acetokinase

1 Images
SDS-PAGE - Recombinant E. coli Acetate Kinase protein (Tagged) (AB268312)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant E. coli Acetate Kinase protein (Tagged) (AB268312)

SDS-PAGE analysis of ab268312.

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

GST tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

P0A6A3

不含动物源

No

不含载体蛋白

No

种属

Escherichia coli K-12

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.004% EGTA, 0.003% EDTA, 0.002% PMSF

storage-buffer

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

序列信息

[{"linker":null,"sequence":"MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":400,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0A6A3","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Acetate kinase sometimes referred to as ACK or ACKA is an enzyme that catalyzes the transfer of a phosphate group from ATP to acetate producing acetyl phosphate. This reaction is a reversible process. Acetate kinase has a molecular mass of approximately 43 kDa. It is expressed in various prokaryotic organisms including bacteria such as Escherichia coli. Additionally some eukaryotic organisms particularly certain fungi show expression of acetate kinase although with some differences in regulation and function compared to their prokaryotic counterparts.
Biological function summary

Acetate kinase plays a role in bacterial energy metabolism. It assists in the regulation of the acetyl-CoA pool an important molecule in energy production pathways. Acetate kinase does not function as part of a larger enzyme complex; rather it operates independently. In prokaryotes it participates in energy conservation by converting acetyl phosphate into ATP via substrate-level phosphorylation therefore influencing cellular energy dynamics. The enzyme's activity directly impacts acetate metabolism and the balance between anabolism and catabolism within the cell.

Pathways

Acetate kinase participates in the prokaryotic phosphotransacetylase-acetate kinase pathway which is integral to acetate metabolism and cellular respiration. This pathway collaborates closely with the TCA cycle by interacting with acetyl-CoA influencing the energy supply for cell processes. Proteins involved in related pathways include phosphotransacetylase which directly precedes acetate kinase in the pathway. Together they form a link between acetyl-CoA metabolism and energy generation impacting bacterial growth and survival.

Researchers have associated acetate kinase with conditions related to bacterial infections. In pathogenic bacteria such as Salmonella enterica acetate kinase contributes to virulence by adapting metabolic responses during infection aiding bacterial survival in host environments. Furthermore diseases linked to dysbiosis in the gut might be affected by aberrant bacterial metabolism involving acetate kinase as it alters microbial energy production. This relationship may involve dysregulated expression or activity of acetate kinase potentially impacting the overall balance of microbial populations and their interactions with host tissues.

特殊说明

形式

Liquid

常规信息

功能

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. The main pathway for acetate production during exponential phase (PubMed : 16080684).

序列相似性

Belongs to the acetokinase family.

产品实验方案

靶点信息

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. The main pathway for acetate production during exponential phase (PubMed : 16080684).
See full target information ackA

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