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AB51121

重组 DnaK 蛋白 (Tag Free)

Recombinant DnaK protein (Tag Free)

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(2 Publications)

Recombinant DnaK protein (Tag Free) is a Escherichia coli K-12 Full Length protein, in the 1 to 638 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

查看别名

groP, grpF, seg, b0014, JW0013, dnaK, Chaperone protein DnaK, HSP70, Heat shock 70 kDa protein, Heat shock protein 70

1 Images
SDS-PAGE - Recombinant DnaK protein (Tag Free) (AB51121)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant DnaK protein (Tag Free) (AB51121)

15% SDS-PAGE

关键信息

纯度

>85% SDS-PAGE

表达系统

Escherichia coli

标签

Tag free

应用

SDS-PAGE

applications

生物活性

No

访问

P0A6Y8

不含动物源

No

不含载体蛋白

No

种属

Escherichia coli K-12

存储溶液

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

序列信息

[{"linker":null,"sequence":"MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":638,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0A6Y8","tags":[]}]

性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False

特殊说明

形式

Liquid

常规信息

功能

Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.

序列相似性

Belongs to the heat shock protein 70 family.

翻译后修饰

Autophosphorylated; GrpE inhibits the autophosphorylation.. Can be UMPylated on a tyrosine residue by SelO/YdiU, probably under stress conditions.

产品实验方案

靶点信息

Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.
See full target information dnaK

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33858942

2021

Killed whole-genome reduced-bacteria surface-expressed coronavirus fusion peptide vaccines protect against disease in a porcine model.

Applications

Unspecified application

Species

Unspecified reactive species

Denicar Lina Nascimento Fabris Maeda,Debin Tian,Hanna Yu,Nakul Dar,Vignesh Rajasekaran,Sarah Meng,Hassan M Mahsoub,Harini Sooryanarain,Bo Wang,C Lynn Heffron,Anna Hassebroek,Tanya LeRoith,Xiang-Jin Meng,Steven L Zeichner

Molecular cell 70:614-627.e7 PubMed29754824

2018

CnoX Is a Chaperedoxin: A Holdase that Protects Its Substrates from Irreversible Oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Camille V Goemans,Didier Vertommen,Rym Agrebi,Jean-François Collet
View all publications

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