重组Cyclophilin B蛋白(ab53870)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组Cyclophilin B蛋白
参阅全部 Cyclophilin B 蛋白酶 -
生物活性
Specific activity is > 1,500nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
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纯度
> 95 % SDS-PAGE.
This product was expressed in E.coli and purified by using conventional chromatography techniques. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab53870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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质谱法
MALDI-TOF -
形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0077% DTT, 0.242% Tris, 10% Glycerol (glycerin, glycerine), 0.116% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- AA408962
- AA553318
- AI844835
see all -
功能
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
疾病相关
Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients. -
序列相似性
Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
Contains 1 PPIase cyclophilin-type domain. -
细胞定位
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
图片
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SDS-PAGE - Recombinant Cyclophilin B protein (ab53870)
ab53870 on 15% SDS-PAGE (3μg)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab53870 被引用在 1 文献中.
- Watanabe A et al. CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells. J Virol 84:4183-93 (2010). PubMed: 20147391