重组噬菌体P1 Cre recombinase蛋白(ab134845)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组噬菌体P1 Cre recombinase蛋白 -
纯度
> 90 % SDS-PAGE.
ab134845 is chromatographically purified. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Bacteriophage P1 -
序列
MASMTGGQQM GRGHHHHHHG NLYFQGGEFP PKKKRKVSNL LTVHQNLPAL PVDATSDEVR KNLMDMFRDR QAFSEHTWKM LLSVCRSWAA WCKLNNRKWF PAEPEDVRDY LLYLQARGLA VKTIQQHLGQ LNMLHRRSGL PRPSDSNAVS LVMRRIRKEN VDAGERAKQA LAFERTDFDQ VRSLMENSDR CQDIRNLAFL GIAYNTLLRI AEIARIRVKD ISRTDGGRML IHIGRTKTLV STAGVEKALS LGVTKLVERW ISVSGVADDP NNYLFCRVRK NGVAAPSATS QLSTRALEGI FEATHRLIYG AKDDSGQRYL AWSGHSARVG AARDMARAGV SIPEIMQAGG WTNVNIVMNY IRNLDSETGA MVRLLEDGDL EESGGGGSPG RRRRRRRRRR R -
氨基酸
2 to 343 -
额外的序列信息
N-terminal T7 tag/His/TEV cleavage site tag and C-terminal 11R tag.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab134845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
Applications:
1. Protein intracellular transduction for in vitro Cre-loxP recombination.
2. Negative control for 11R-Tag human transcription factor mediated cell differentiation assay in vitro.
Functional Assay: Mix 1µg Cre-11R protein with 2ul ProFectinTM by following ProFectinTM manufacture suggested protocol, and incubate transfection mixture on 0.25 x106 293-LoxP-eGFP cell line / well (6 well plate) for 30 min in serum free medium, will achieve > 35% transduction efficiency. (Positive for GFP cells).
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 0.58% Sodium chloride, 0.08% Potassium chloride, 10% Glycerol
常规信息
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别名
- Cre
- Cyclization recombinase
- Recombinase cre
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相关性
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab134845 被引用在 1 文献中.
- Chkaiban L et al. Assembly of Long-Adapter Single-Strand Oligonucleotide (LASSO) Probes for Massively Parallel Capture of Kilobase Size DNA Targets. Curr Protoc 1:e278 (2021). PubMed: 34807521