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AB204629

重组Arginine deiminase蛋白

Recombinant Arginine deiminase protein

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Recombinant Arginine deiminase protein is a Mycoplasma arginini Full Length protein, in the 2 to 410 aa range, expressed in Escherichia coli, with >97%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

查看别名

Arginine deiminase, ADI, Arginine dihydrolase, AD, arcA

关键信息

纯度

>97% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

Escherichia coli

标签

Tag free

应用

FuncS, HPLC, SDS-PAGE

applications

生物活性

Yes

生物学活性

Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.

访问

P23793

不含动物源

No

不含载体蛋白

No

种属

Mycoplasma arginini

复溶

Reconstitute in water

存储溶液

Constituents: 0.03278% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

序列信息

[{"sequence":"SVFDSKFKGIHVYSEIGELESVLVHEPGREIDYITPARLDELLFSAILESHDARKEHKQFVAELKANDINVVELIDLVAETYDLASQEAKDKLIEEFLEDSEPVLSEEHKVVVRNFLKAKKTSRELVEIMMAGITKTDLGIEADHELIVDPMPNLYFTRDPFASVGNGVTIHYMRYKVRQRETLFSRFVFSNHPKLINTPWYYDPSLKLSIEGGDVFIYNNDTLVVGVSERTDLQTVTLLAKNIVANKECEFKRIVAINVPKWTNLMHLDTWLTMLDKDKFLYSPIANDVFKFWDYDLVNGGAEPQPVENGLPLEGLLQSIINKKPVLIPIAGEGASQMEIERETHFDGTNYLAIRPGVVIGYSRNEKTNAALEAAGIKVLPFHGNQLSLGMGNARCMSMPLSRKDVKW","proteinLength":"Full Length","predictedMolecularWeight":"46.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":410,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P23793","tags":[]}]

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle
True

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Arginine deiminase also known as protein arginine deiminase or deiminase is an enzyme that converts arginine into citrulline and ammonia by deimidation. The enzyme shows molecular mass around 46 kDa. It can be expressed in a variety of tissues with significant presence observed in bacterial species and in several types of human cells. This enzyme plays an important role in regulating the availability of arginine which is essential for protein synthesis and other metabolic processes.
Biological function summary

The regulation of arginine availability has far-reaching effects on cellular functions. Arginine deiminase takes part in modulating immune responses and can influence nitric oxide production essential in inflammation and cell signaling. This enzyme often functions within a larger enzymatic complex. Its activity helps control cellular proliferation and has implications in both energy metabolism and immune modulation.

Pathways

Converting arginine has significant impact on metabolic and immune pathways. Arginine deiminase has been linked to the urea cycle influencing nitrogen excretion and metabolism. Moreover the enzyme aligns with pathways involved in mTOR signaling a pathway important for cell growth and proliferation. Arginase is another protein associated within these pathways often acting in concert with arginine deiminase to regulate arginine levels.

The role of arginine deiminase relates closely to cancer and immune system dysfunctions. In cancer particularly hepatocellular carcinoma the enzyme's ability to deplete arginine can starve tumor cells reducing their proliferation. The enzyme also connects with immune checkpoints influencing disorders like rheumatoid arthritis by regulating inflammation. Protein interactions with ornithine another amino acid derivative through arginase activity also play role in these diseases.

特殊说明

形式

Lyophilized

附加说明

>97 % by HPLC.

常规信息

序列相似性

Belongs to the arginine deiminase family.

产品实验方案

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