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Native金黄色葡萄球菌蛋白A (FITC) (ab7455)

Submit a review Q&A (3)References (2)

Key features and details

  • Expression system: Native
  • Purity: > 95% n/a
  • Suitable for: Flow Cyt, Immunomicroscopy, WB, Dot blot

描述

  • 产品名称

    Native金黄色葡萄球菌蛋白A (FITC)
    参阅全部 Protein A 蛋白酶

  • 纯度

    > 95 % n/a.
    This product was prepared from chromatographically pure Protein A.

  • 表达系统

    Native

  • Accession

    P38507

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Native

    • 种属

      Staphylococcus aureus

    • 预测分子量

      56 kDa

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

技术指标

Our Abpromise guarantee covers the use of ab7455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Flow Cytometry

    Immunomicroscopy

    Western blot

    Dot blot

  • 形式

    Liquid

  • 补充说明

    Molecular Weight (protein A): 42,000 daltons

    Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C.

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, BSA

常规信息

  • 别名

    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A

  • 相关性

    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

  • 细胞定位

    Secreted; cell wall.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab7455？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7455 被引用在 2 文献中.

  • Nepal S  et al. Injectable phase-separated tetra-armed poly(ethylene glycol) hydrogel scaffold allows sustained release of growth factors to enhance the repair of critical bone defects. Regen Ther 25:24-34 (2024). PubMed: 38108043
  • He B  et al. Group A Rotaviruses in Chinese Bats: Genetic Composition, Serology, and Evidence for Bat-to-Human Transmission and Reassortment. J Virol 91:N/A (2017). IF . PubMed: 28381569

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Protein G-FITC conjugated (ab7455)
Protein A- FITCconjugated (ab7459)
Do you have APC and PE conjugated Protein G and A products?
Do you have recombinant anti human IgG1?

Read More
Answer

Thank you very much for having patience.

My colleague has tried to source these products form our collaborator supplier however due to non availability of these product we are unable to add these in catalogue.

You can however use the following kits to conjugate the protein yourselves. Please check the following products

ab102851, ab102849, ab102850, ab102848

ab102912, ab102870

Please click the below given link for full list;

https://www.abcam.com/index.html?pageconfig=resource&rid=13148

We unfortunately do not have recombinant anti human IgG1 antibody available.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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Question

Protein G-FITC conjugated (ab7455)
Protein A- FITCconjugated (ab7459)
Do you have APC and PE conjugated Protein G and A products?
Do you have recombinant anti human IgG1?

Read More
Answer

Thank you for contacting us.

I can confirm that I have forwarded your inquiry to my colleague, who is protein product manager at Abcam. She is now looking into the requested product line and will be contacting me with updates soon.

I will get back to you, as soon as I get a reply from her.

Thank you very much for having patience!

Read More

Question

Can you please provide a certificate of origin for this product, or other regulatory information about possible contamination of this product with Mad Cow Disease?

Read More
Answer

Thank you for contacting us. To my knowledge we do not have a prepared certificate of origin document for this protein. However, this product was derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. I hope this helps, please let me know if you need any other information.

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