Native金黄色葡萄球菌蛋白A (Alkaline Phosphatase) (ab7345)
Key features and details
- Expression system: Native
- Purity: > 95% Protein A purified
- Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr
描述
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产品名称
Native金黄色葡萄球菌蛋白A (Alkaline Phosphatase)
参阅全部 Protein A 蛋白酶 -
纯度
> 95 % Protein A purified. -
表达系统
Native -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Staphylococcus aureus -
预测分子量
56 kDa
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偶联物
Alkaline Phosphatase
技术指标
Our Abpromise guarantee covers the use of ab7345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
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形式
Liquid -
补充说明
Protein A Concentration: 1.0 mg/ml (by UV absorbance at 280 nm) This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the stock concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1.5% BSA
BSA is IgG and Protease free
常规信息
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别名
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
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相关性
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
细胞定位
Secreted; cell wall.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab7345 尚未被引用在任何文献中。