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Native金黄色葡萄球菌蛋白A (ab7399)

Submit a review Q&A (4)References (4)

Key features and details

  • Expression system: Native
  • Purity: > 95% n/a
  • Suitable for: ELISA, WB

描述

  • 产品名称

    Native金黄色葡萄球菌蛋白A
    参阅全部 Protein A 蛋白酶

  • 纯度

    > 95 % n/a.
    Protein A is chromatographically pure and shows predominantly a single band by SDS-PAGE.

  • 表达系统

    Native

  • Accession

    P38507

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Native

    • 种属

      Staphylococcus aureus

    • 预测分子量

      56 kDa

技术指标

Our Abpromise guarantee covers the use of ab7399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    Western blot

  • 形式

    Liquid

  • 补充说明

    Molecular Weight: 42,000 daltons

    Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

常规信息

  • 别名

    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A

  • 相关性

    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

  • 细胞定位

    Secreted; cell wall.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (4)

发表研究结果有使用 ab7399？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7399 被引用在 4 文献中.

  • Zhang P  et al. Evanescent scattering imaging of single protein binding kinetics and DNA conformation changes. Nat Commun 13:2298 (2022). PubMed: 35484120
  • Romih T  et al. The Effect of Preconditioning Strategies on the Adsorption of Model Proteins onto Screen-Printed Carbon Electrodes. Sensors (Basel) 22:N/A (2022). PubMed: 35684806
  • Hsu MN  et al. Smart Hydrogel Microfluidics for Single-Cell Multiplexed Secretomic Analysis with High Sensitivity. Small 14:e1802918 (2018). PubMed: 30334375
  • Hong LTA  et al. An injectable hydrogel enhances tissue repair after spinal cord injury by promoting extracellular matrix remodeling. Nat Commun 8:533 (2017). PubMed: 28912446

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Question

can u tell me if it has a histag




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Answer

Hello! It is a native protein with no His tag. Please let me know if I can be of further assistance.

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Question



Inquiry: Hey I have a question about protein A protein (ab7399) - does it have a histag? Or no? how was the purification done then? Thanks.

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Answer

Thank you for contacting us.


Our Protein A is purified using a process that is considered proprietary so unfortunately I am unable to provide any details. Please let me know if I can be of further assistance.

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Question

- protein G (full length)
- protein G (full length) + His tag ....... ab7458.

- protein A (full length) ....... ab117657.
- protein A (full length) + His tag

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Answer

J'aimerais vous informer que mes collègues responsables de l'intégration des produits travaillent toujours à la recherche d'un collaborateur qui pourrait nous fournir :
- une protéine G (full length sans tag) en complément de la référence ab7458 (protéine G, full length, His tag),
- une protéine A (full length avec His-tag) en complément de la référence ab117657 (protéine A, full length, sans tag)

Je vous contacterai de nouveau avec les informations que mes collègues m'auront fournies.

Merci pour votre patience et bon week-end.

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Question

Dear technical support team: This customer raises an enquiry about ab7399 (Protein A protein) and ab84187 (Protein A protein), she wants to know which one is more suitable to bind with ab9582 (Anti-hCG beta antibody [5H4-E2]), could you please help this customer to solve the problem? Thanks for your kindly help Best regards

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Answer

Thank you for your enquiry and your interest in our products.

I can confirm that ab9582 (Anti-hCG beta antibody [5H4-E2]), has not been tested for IP yet, though the isotype has been determined (IgG1).

Normally, we would suggest using Protein G for mouse IgG1 antibodies; whilst for moue IgG2a and mouse IgG2b can be used with both Protein A and G. Please could you advise your customer to take a look at this site for further information:

https://www.abcam.com/index.html?pageconfig=resource&rid=11385

I hope this helps and if I can assist further, please do not hesitate to contact me.

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