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Native蛋白G (Alkaline Phosphatase) (ab7461)

Submit a review Q&A (1)References (1)

Key features and details

  • Expression system: Native
  • Purity: > 98% Protein G purified
  • Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr

描述

  • 产品名称

    Native蛋白G (Alkaline Phosphatase)
    参阅全部 Protein G 蛋白酶

  • 纯度

    > 98 % Protein G purified.

  • 表达系统

    Native

  • Accession

    P19909

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Native

    • 种属

      Streptococcus

    • 预测分子量

      63 kDa

  • 偶联物

    Alkaline Phosphatase

技术指标

Our Abpromise guarantee covers the use of ab7461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

  • 形式

    Liquid

  • 补充说明

    Molecular Weight (protein G): 33,000 daltons Extinction Coefficient: 1% at 280 nm is 9.5(E0.1% of 9.5)
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride

常规信息

  • 别名

    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all

  • 相关性

    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

  • 细胞定位

    Cell Wall and Secreted

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab7461？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7461 被引用在 1 文献中.

  • Zheng W & Yu A EZH2-mediated suppression of lncRNA-LET promotes cell apoptosis and inhibits the proliferation of post-burn skin fibroblasts. Int J Mol Med 41:1949-1957 (2018). PubMed: 29393360

客户评价及客户问答

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Question

Can you please provide information regarding the following questions?

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Answer

Thank you for your enquiry. Here are the answers to your questions. 1) What is the format of this protein G (e.g Fc region only, albumin domain only) Answer: Recombinant Protein G containing IgG binding domain 2) Do you have any reference on the use of this product? Answer: No references available 3) What is the affinity constant, dissociative rate constant ? Do you have any data for this? Answer: The affinity constant is not determined 4) How much is the relative specific activity of AP? Please also explain about the activity and use of AP for conjugation. Answer: The specific activity of the AP is not determined after conjugation. Before conjugation, the Alkaline phosphatase has an activity between 7000 and 8000 units per mg. Unit definition is amount of enzyme causing hydrolysis of one micromole of p-nitrophenylphosphate per minute at 25 degrees C pH 9.6. 5) What is the ratio of Protein G to AP? Answer: 3-5 moles protein G per mole of Alkaline Phosphatase

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