Native人Prethrombin 2蛋白(ab62535)
Key features and details
- Expression system: Native
- Purity: > 95% Ion Exchange Chromatography
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
Native人Prethrombin 2蛋白 -
生物活性
Activity was measured by clotting and/or chromogenic substrate assay, following conversion of prothrombin to thrombin. -
纯度
> 95 % Ion Exchange Chromatography.
Prepared from fresh frozen human plasma. Purity was determined by SDS-PAGE analysis. -
表达系统
Native -
蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Human
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技术指标
Our Abpromise guarantee covers the use of ab62535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol, 50% Water
50% H2OThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Prothrombin
- Prothrombin precursor
- PT
- Serine protease
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相关性
Prethrombin 2, the smallest single-chain immediate precursor of a-thrombin (corresponding to residues Thr272 to Glu579 in prothrombin), has one glycosylation site at Asn373 and four disulfide bonds, Cys293-Cys439, Cys348-Cys364, Cys493-Cys507, and Cys521-Cys551. alpha-Thrombin can be derived from prothrombin by two pathways, each of which generates different intermediate products. In one pathway, human prothrombin is converted relatively slowly to alpha-thrombin in the presence of factor Xa and Ca2+ ions by initial cleavage at residue (Arg) 271/(Thr) 272 with consequent production of fragment 1.2 and prethrombin 2. Prethrombin 2 is then cleaved at R 320 with consequent generation of alpha-thrombin. In the other pathway, initial cleavage at R 320 yields an intermediate product, meizothrombin. -
细胞定位
Secreted, extra cellular space
图片
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SDS-PAGE - Native human Prethrombin 2 protein (ab62535)Novex 4-12% Bis-Tris
1 µg per lane
MOPS
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab62535 被引用在 1 文献中.
- Jonebring A et al. Expression and characterization of recombinant ecarin. Protein J 31:353-8 (2012). PubMed: 22528138