Native人Plasmin蛋白(Active)
Native human Plasmin protein (Active)
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(3 Publications)
Native human Plasmin protein (Active) is a Human Full Length protein with >=95% purity and suitable for Functional studies and SDS-PAGE.The predicted molecular weight of ab90928 native protein is 81 kDa.
- Save time and ensure accurate results- use our Plasmin protein as a control
- Optimal protein bioactivity and stability
查看别名
Plasminogen, PLG
- SDS-PAGE
Unknown
SDS-PAGE - Native human Plasmin protein (Active) (AB90928)
4-12% Bis-Tris NuPAGE gel.
Lane 1 : Plasmin - 5 μg (reduced/ heated)
Lane 2 : Plasmin - 10 μg (reduced/ heated)
Lane 3 : Plasmin - 20 μg (reduced/ heated)
Lane 4 : LMW Standard
Lane 5 : Plasmin - 5 μg (non-reduced/ no heat)
Lane 6 : Plasmin - 10 μg (non-reduced/ no heat)
Lane 7 : Plasmin - 20 μg (non-reduced/ no heat)
反应性数据
产品详情
The Native human Plasmin protein (Active) ab90928 is prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Ensure the validity of your result using our native human Plasmin protein as a positive control in SDS-PAGE. The bioactive native human Plasmin ab90928 has been prepared by using streptokinase to activate human plasma plasminogen.
Check out our protein gel staining guide for SDS-PAGE here
Protein Determination: Extinction Coefficient (ε) 0.1% at 280 nm 1cm pathway = 1.71
序列信息
性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme plasmin degrades fibrin in blood clots a critical function in the process of clot resolution. It belongs to the family of serine proteases and does not form part of a larger protein complex. The activity of plasmin is modulated by inhibitors like alpha-2-antiplasmin which bind to plasmin and prevent excessive degradation of fibrin. Bovine plasmin shares similar functions and serves as a model for studying human plasmin activity. Researchers often measure plasmin activity through specific assays to understand changes in fibrinolytic activity within the body.
Pathways
Plasmin plays an integral role in the fibrinolytic pathway and is also involved in tissue remodeling and wound healing. The fibrinolytic pathway centers on the balance of plasminogen activation and inhibition which directly influences thrombolysis. Proteins such as tPA and uPA facilitate the conversion of plasminogen to plasmin and are tightly regulated to ensure proper physiological function. In the context of wound healing plasmin helps activate matrix metalloproteases leading to extracellular matrix breakdown and cell migration.
特殊说明
形式
Lyophilized
常规信息
功能
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.. (Microbial infection) ENO/enoloase from parasite P.falciparum (strain NF54) interacts with PLG present in the mosquito blood meal to promote the invasion of the mosquito midgut by the parasite ookinete (PubMed : 21949403). The catalytic active form, plasmin, is essential for the invasion of the mosquito midgut (PubMed : 21949403).. (Microbial infection) Binds to OspC on the surface of B.burgdorferi cells, possibly conferring an extracellular protease activity on the bacteria that allows it to traverse host tissue.
序列相似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
翻译后修饰
N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).. In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.. (Microbial infection) The Y.pestis Pla protein cleaves between Arg-580 and Val-581, generating plasmin which facilitates bacterial migration and infection (PubMed:22645135).
靶点信息
文献 (3)
Recent publications for all applications. Explore the full list and refine your search
Translational vision science & technology 12:23 PubMed37074731
2023
Applications
Unspecified application
Species
Unspecified reactive species
Nature chemical biology 18:1125-1134 PubMed35941237
2022
Applications
Unspecified application
Species
Unspecified reactive species
Human molecular genetics 25:328-39 PubMed26604146
2015
Applications
Unspecified application
Species
Unspecified reactive species
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