Native人Fibronectin蛋白(ab80021)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
描述
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产品名称
Native人Fibronectin蛋白
参阅全部 Fibronectin 蛋白酶 -
纯度
> 95 % SDS-PAGE.
Purified from human plasma -
表达系统
Native -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Human -
额外的序列信息
Molecular weight: the MW of the hetrodimer is 550 kDa
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab80021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 0.476% HEPES, 0.87% Sodium chloride -
复溶Reconstitute with 1.0 milliliters of water to achieve 2.0 mg/mL. Human Fibronectin is known for its solubility issues. The best way to get this material into solution, after reconstitution, is to incubate in a 37ºC water bath for at least 30 minutes. Typically this is sufficient, however a small amount of un-dissolved material may remain. If this is the case, a few gentle aspirations with a pipette is recommended.
常规信息
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别名
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see all -
功能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
组织特异性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
疾病相关
Glomerulopathy with fibronectin deposits 2 -
序列相似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
发展阶段
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻译后修饰
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
图片
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SDS-PAGE - Native Human Fibronectin protein (ab80021)
Gel: Novex 4-12% Bis-Tris
Load Load: Human Fibronectin, 1 µg per lane in Non-reducing (NR) and reducing (R) conditions.
Buffer: MOPS
MW Ladder: Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab80021 尚未被引用在任何文献中。
