Native人Collagen III蛋白(ab7535)
Key features and details
- Expression system: Native
- Suitable for: WB, SDS-PAGE, IP, ELISA
描述
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产品名称
Native人Collagen III蛋白
参阅全部 Collagen III 蛋白酶 -
表达系统
Native -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Human
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相关产品
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Positive Controls
技术指标
Our Abpromise guarantee covers the use of ab7535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
Immunoprecipitation
ELISA
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 3% Acetic acid
常规信息
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别名
- Alpha 1 type III collagen
- Alpha1 (III) collagen
- CO3A1_HUMAN
see all -
功能
Collagen type III occurs in most soft connective tissues along with type I collagen. -
疾病相关
Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. -
序列相似性
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
翻译后修饰
Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
图片
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Western blot - Native Human Collagen III protein (ab7535)Image courtesy of Michaela Leyh by Abreview.All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution
Lane 1 : plus ab7535 reduced and denatured
Lane 2 : plus ab7535 native
Secondary
All lanes : Goat anti-mouse-HRP at 1/2000 dilution
Predicted band size: 139 kDa
The protein can be used reduced + denaturated (band size ~130 kDa) or native (two bands ~ 130kDa and ~300kDa) The native protein gives a brighter signal.PH of the protein lysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot. -
SDS-PAGE - Native Human Collagen III protein (ab7535)SDS-PAGE analysis of ab7535.
Lane 1: ab7535 Reduced 1 µg.
Lane 2: Pre-stained Molecular Weight Marker.
Lane 3: ab7535 Non-Reduced 1 µg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab7535 被引用在 6 文献中.
- Jensen C et al. Autoreactivity against Denatured Type III Collagen Is Significantly Decreased in Serum from Patients with Cancer Compared to Healthy Controls. Int J Mol Sci 24:N/A (2023). PubMed: 37108227
- Di Francesco S et al. Red grape (Vitis vinifera L.) flavonoids down-regulate collagen type III expression after UV-A in primary human dermal blood endothelial cells. Exp Dermatol 27:973-980 (2018). PubMed: 29742305
- Wang Z et al. Extracellular matrix promotes proliferation, migration and adhesion of airway smooth muscle cells in a rat model of chronic obstructive pulmonary disease via upregulation of the PI3K/AKT signaling pathway. Mol Med Rep 18:3143-3152 (2018). PubMed: 30066869
- Kehlet SN et al. A fragment of SPARC reflecting increased collagen affinity shows pathological relevance in lung cancer - implications of a new collagen chaperone function of SPARC. Cancer Biol Ther 19:904-912 (2018). PubMed: 30067436
- Barbaglio A et al. Ultrastructural and biochemical characterization of mechanically adaptable collagenous structures in the edible sea urchin Paracentrotus lividus. Zoology (Jena) 118:147-60 (2015). PubMed: 25958104
- Syed F et al. Fibroblasts from the growing margin of keloid scars produce higher levels of collagen I and III compared with intralesional and extralesional sites: clinical implications for lesional site-directed therapy. Br J Dermatol 164:83-96 (2011). PubMed: 20849516