Native Clostridium difficile Toxin B蛋白(ab124001)
Key features and details
- Expression system: Native
- Purity: > 95% Affinity purified
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
Native Clostridium difficile Toxin B蛋白
参阅全部 Clostridium difficile Toxin B 蛋白酶 -
生物活性
Measured by vercytotoxicity assay with 2 pg/ml as lowest point at which rounded cells are observed.
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纯度
> 95 % Affinity purified.
Clostridium difficile Toxin B has been highly purified. -
表达系统
Native -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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预测分子量
270 kDa -
氨基酸
2 to 2366
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab124001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Functional Studies Protocol
1.1 Vero cells are plated out the night before on a 96-well plate at a concentration of 20K cells per well (in 100ml), then incubated at 37°C, 5% CO2. The following day they should be lightly confluent.
1.2 Lyophilised toxin/toxoid is resuspended as per the instructions in the certificate of analysis, and diluted in duplicate to 0.1mg/ml in DMEM (200ml total) without FBS in the first well of a non-TC-treated 96-well plate. A serial 5-fold dilution is performed (40ul in 160ul) in the same media using a multichannel for 11 dilutions in total, leaving the 12th dilution as a media control.
1.3 20ul of each dilution is then pipetted onto each well of the pre-prepared Vero cells and then incubated at 37°C, 5% CO2 for 24 hours.
1.4 The following day, the cells are examined using an inverted phase contrast tissue culture microscope. Cells with active toxin on should be rounded, cells without significant toxicity should look like the control cells. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.00
Constituents: 1.19% HEPES, 5% Sucrose, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute in 250µl sterile distilled water. Swirl or pipette gently to mix. Vortexing promotes aggregation. Store the lyophilised vial between +2°C to +8°C for up to 1 year from date of receipt. Store the reconstituted liquid between +2°C to +8°C for up to a month. Reconstituted liquid can be frozen immediately and stored at -80°C to extend stability to 1 year.
常规信息
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别名
- C. difficile Toxin B
- Clostridium difficile Toxin B
- Cytotoxin B
see all -
相关性
Clostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli. -
细胞定位
Secreted
图片
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SDS-PAGE - Native Clostridium difficile Toxin B protein (ab124001)SDS-PAGE showing ab124001 at 270 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab124001 被引用在 7 文献中.
- Chirita D et al. Functional Assessment of Disease-Associated Pyrin Variants. Methods Mol Biol 2523:179-195 (2022). PubMed: 35759198
- Magnotti F et al. Steroid hormone catabolites activate the pyrin inflammasome through a non-canonical mechanism. Cell Rep 41:111472 (2022). PubMed: 36223753
- Li S et al. ASC deglutathionylation is a checkpoint for NLRP3 inflammasome activation. J Exp Med 218:N/A (2021). PubMed: 34342641
- Magnotti F et al. Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients. EMBO Mol Med 11:e10547 (2019). PubMed: 31589380
- Hartley-Tassell LE et al. Lectin activity of the TcdA and TcdB toxins of Clostridium difficile. Infect Immun N/A:N/A (2018). PubMed: 30530621
- Moghaddas F et al. A novel Pyrin-Associated Autoinflammation with Neutrophilic Dermatosis mutation further defines 14-3-3 binding of pyrin and distinction to Familial Mediterranean Fever. Ann Rheum Dis 76:2085-2094 (2017). PubMed: 28835462
- Hutton ML et al. Bovine antibodies targeting primary and recurrent Clostridium difficile disease are a potent antibiotic alternative. Sci Rep 7:3665 (2017). Neutralising ; Clostridium difficile . PubMed: 28623367