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AB15581

Ki67多肽

Ki67 peptide

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(6 Publications)

Ki67 peptide is a Synthetic blocking peptide. >90% and suitable for BL.

查看别名

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

1 Images
Western blot - Ki67 peptide (AB15581)
  • WB

Project

Western blot - Ki67 peptide (AB15581)

Observed band sizes : 345kDa, 395kDa

All lanes:

Western blot - Anti-Ki67 antibody (<a href='/products/primary-antibodies/ki67-antibody-ab15580'>ab15580</a>) at 1 µg/mL

All lanes:

Hela whole cell lysate at 20 µg

Secondary

All lanes:

Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

false

关键信息

纯度

>90% HPLC

标签

Tag free

应用

BL

applications

生物活性

No

访问

P46013

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 6.75 Constituents: 0.87% Sodium chloride, 0.714% HEPES, 0.0584% EDTA, 0.001% Sorbitan monolaurate, ethoxylated

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg/mL", "notes":"<p></p>" } } }
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产品详情

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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序列信息

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"P46013","tags":[]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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特殊说明

形式

Liquid

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常规信息

功能

The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed : 27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed : 27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed : 10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed : 24867636). This supplementary information is collated from multiple sources and compiled automatically.

翻译后修饰

Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed : 27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed : 27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed : 10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed : 24867636). This supplementary information is collated from multiple sources and compiled automatically.
See full target information MKI67
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 32:101851 PubMed40487169

2025

Integrating gene demethylation and immune modulation: PD-1 nanovesicles as a dual-action therapy for NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhang,Yuan Liang,Shiyang Deng,Jiang Du

Neoplasma 70:272-286 PubMed37226932

2023

NUCKS1 promotes the progression of colorectal cancer via activating PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liao-Liao Zhu,Jing-Jie Shi,Yong-Dong Guo,Cheng Yang,Rong-Lin Wang,Shan-Shan Li,Dong-Xue Gan,Pei-Xiang Ma,Jun-Qiang Li,Hai-Chuan Su

Science advances 6:eaay6324 PubMed31998844

2020

Microglia response following acute demyelination is heterogeneous and limits infiltrating macrophage dispersion.

Applications

Unspecified application

Species

Unspecified reactive species

Jason R Plemel,Jo Anne Stratton,Nathan J Michaels,Khalil S Rawji,Eric Zhang,Sarthak Sinha,Charbel S Baaklini,Yifei Dong,Madelene Ho,Kevin Thorburn,Timothy N Friedman,Sana Jawad,Claudia Silva,Andrew V Caprariello,Vahid Hoghooghi,Julie Yue,Arzina Jaffer,Kelly Lee,Bradley J Kerr,Raj Midha,Peter K Stys,Jeff Biernaskie,V Wee Yong

The Journal of comparative neurology 527:1654-1674 PubMed30552685

2018

Cortical interlaminar astrocytes across the therian mammal radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Falcone,Marisol Wolf-Ochoa,Sarwat Amina,Tiffany Hong,Gelareh Vakilzadeh,William D Hopkins,Patrick R Hof,Chet C Sherwood,Paul R Manger,Stephen C Noctor,Verónica Martínez-Cerdeño

Journal of vascular surgery 67:933-944.e3 PubMed29477204

2018

MicroRNA-155 promotes neointimal hyperplasia through smooth muscle-like cell-derived RANTES in arteriovenous fistulas.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wang,Peng Deng,Yuan Sun,Ping Ye,Anchen Zhang,Chuangyan Wu,Zhang Yue,Zhaolei Chen,Jiahong Xia

Clinical cancer research : an official journal of the American Association for Cancer Research 16:212-25 PubMed20008843

2009

Quantified tumor t1 is a generic early-response imaging biomarker for chemotherapy reflecting cell viability.

Applications

BL

Species

Unspecified reactive species

Paul M J McSheehy,Claudia Weidensteiner,Catherine Cannet,Stephane Ferretti,Didier Laurent,Stephan Ruetz,Michael Stumm,Peter R Allegrini
View all publications
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搭配使用产品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB29

Anti-PCNA antibody [PC10]

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PCNA antibody [PC10] (AB29)

  • 5
  • 72
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6

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