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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments

人Nestin多肽(ab19375)

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Human Nestin peptide (ab19375)

    Key features and details

    • Purity: > 70% HPLC
    • Suitable for: Blocking

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    描述

    • 产品名称

      人Nestin多肽
    • 纯度

      > 70 % HPLC.
      70 - 90% by HPLC
    • Accession

      P48681
    • 无动物成分

      No
    • 性质

      Synthetic
      • 种属

        Human

    相关产品

    • Corresponding Antibody

      • Anti-Nestin antibody - Neural Stem Cell Marker (ab92391)

    技术指标

    Our Abpromise guarantee covers the use of ab19375 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Blocking - Blocking peptide for Anti-Nestin antibody - Neural Stem Cell Marker (ab92391)

    • 形式

      Liquid
    • 补充说明

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    常规信息

    • 别名

      • ESTM 46
      • FLJ 21841
      • FLJ21841
      • Intermediate filament protein
      • Nbla00170
      • nes
      • NEST_HUMAN
      • Nestin
      see all
    • 功能

      Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • 组织特异性

      CNS stem cells.
    • 序列相似性

      Belongs to the intermediate filament family.
    • 发展阶段

      Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • 翻译后修饰

      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Target information above from: UniProt accession P48681 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    图片

    • Human Nestin peptide (ab19375)
      Human Nestin peptide (ab19375)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab19375?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab19375 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    I would like to ask you about product: Nestin peptid. Is it possible to use this peptid for in vitro studies as an immunogen for T-cells? Is it sterile? Thank you With regards

    Read More

    Abcam community

    Verified customer

    Asked on Mar 28 2012

    Answer

    Thank you for contacting us.

    The product ab19375 is a 18 amino acid long peptide that can be used to block the antibody ab92391. We have never used this peptide in functional studies e.g. in-vitro or in-vivo. I am sorry we are unable to confirm its use.

    Theoretically, this peptide can't be used because this is not a full length protein. It is just a fragment which may not have the biological activity other than blocking.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Mar 28 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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