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人Histone H3 (unmodified)多肽(ab7228)

  • Datasheet
Submit a review Q&A (1)References (6)

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Human Histone H3 (unmodified) peptide (ab7228)

    Key features and details

    • Purity: > 90% HPLC
    • Suitable for: Blocking, Dot blot

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    描述

    • 产品名称

      人Histone H3 (unmodified)多肽
      参阅全部 Histone H3 蛋白酶
    • 生物活性

      Sequence: ARTKQTARKSTGGKAPGGC Histone H3 N-terminus 1-16,Gly-Gly linker, C-terminal cysteine residue.
    • 纯度

      > 90 % HPLC.

    • Accession

      P68431
    • 无动物成分

      No
    • 性质

      Synthetic
      • 种属

        Human
      • 额外的序列信息

        Synthetic peptide derived from within residues 1 - 100 of Human Histone H3 containing Gly-Gly linker and C-terminal cysteine residue (Note: the amino acid sequence is proprietary).
    • 描述

      人Histone H3 (unmodified )多肽

    技术指标

    Our Abpromise guarantee covers the use of ab7228 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Blocking

      Dot blot

    • 形式

      Lyophilized
    • 补充说明

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at -20°C.

      Information available upon request.

    常规信息

    • 别名

      • H3 histone family member E pseudogene
      • H3 histone family, member A
      • H3/A
      • H31_HUMAN
      • H3F3
      • H3FA
      • Hist1h3a
      • HIST1H3B
      • HIST1H3C
      • HIST1H3D
      • HIST1H3E
      • HIST1H3F
      • HIST1H3G
      • HIST1H3H
      • HIST1H3I
      • HIST1H3J
      • HIST3H3
      • histone 1, H3a
      • Histone cluster 1, H3a
      • Histone H3 3 pseudogene
      • Histone H3.1
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/i
      • Histone H3/j
      • Histone H3/k
      • Histone H3/l
      see all
    • 功能

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 序列相似性

      Belongs to the histone H3 family.
    • 发展阶段

      Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
    • 翻译后修饰

      Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
      Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
      Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
      Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
      Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
      Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
    • 细胞定位

      Nucleus. Chromosome.
    • Target information above from: UniProt accession P68431 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    图片

    • Human Histone H3 (unmodified) peptide (ab7228)
      Human Histone H3 (unmodified) peptide (ab7228)

      To learn more about our protein and peptide range click here.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab7228?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7228 被引用在 6 文献中.

    • Kato S  et al. Gain-of-Function Genetic Alterations of G9a Drive Oncogenesis. Cancer Discov 10:980-997 (2020). PubMed: 32269030
    • Fuchs C  et al. Desmin enters the nucleus of cardiac stem cells and modulates Nkx2.5 expression by participating in transcription factor complexes that interact with the nkx2.5 gene. Biol Open 5:140-53 (2016). PubMed: 26787680
    • Guppy BJ & McManus KJ Mitotic accumulation of dimethylated lysine 79 of histone H3 is important for maintaining genome integrity during mitosis in human cells. Genetics 199:423-33 (2015). Dot blot ; Human . PubMed: 25533199
    • Mulder KW  et al. Regulation of histone H3K4 tri-methylation and PAF complex recruitment by the Ccr4-Not complex. Nucleic Acids Res 35:2428-39 (2007). PubMed: 17392337
    • McManus KJ & Hendzel MJ The relationship between histone H3 phosphorylation and acetylation throughout the mammalian cell cycle. Biochem Cell Biol 84:640-57 (2006). Competitive Binding Assays . PubMed: 16936834
    • Czermin B  et al. Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites. Cell 111:185-96 (2002). PubMed: 12408863

    客户评价及客户问答

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    Question

    I am writing to find out how the specificity of the antibodies I purchased were determined. I bought: Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) I also purchased these peptides: Histone H3 peptide (1-8) - tri methyl K4 (ab1342) Histone H3 peptide (1-10) - di methyl K4 (ab7768) Histone H3 peptide (1-8) - mono methyl K4 (ab1340) Histone H3 peptide (1-16) - unmodified (ab7228)

    Read More

    Abcam community

    Verified customer

    Asked on Apr 06 2005

    Answer

    Specificity of those antibodies was tested by WB of Histone (ab121) (0.5µg per lane) and with blocking peptides (modified and non modified peptides). In each case, blocking was achieved only when the adequate peptide was added.

    Read More

    Abcam Scientific Support

    回复于 Apr 07 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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