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Epigenetics and Nuclear Signaling Histones H3 Methylated

人Histone H3 (tri methyl K9)多肽(ab1773)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (18)

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Dot Blot - Human Histone H3 (tri methyl K9) peptide (ab1773)

    Key features and details

    • Purity: > 90% HPLC
    • Suitable for: Blocking

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    描述

    • 产品名称

      人Histone H3 (tri methyl K9)多肽
      参阅全部 Histone H3 蛋白酶
    • 纯度

      > 90 % HPLC.

    • Accession

      P68431
    • 无动物成分

      No
    • 性质

      Synthetic
      • 种属

        Human
      • 修饰

        tri methyl K10

    相关产品

    • Corresponding Antibody

      • Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)
    • Related Products

      • Anti-Histone H3 (tri methyl K9, phospho S10) antibody (ab5819)

    技术指标

    Our Abpromise guarantee covers the use of ab1773 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Blocking - Blocking peptide for Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)

    • 形式

      Lyophilized
    • 补充说明

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at -20°C.

      Information available upon request.

    常规信息

    • 别名

      • H3 histone family member E pseudogene
      • H3 histone family, member A
      • H3/A
      • H31_HUMAN
      • H3F3
      • H3FA
      • Hist1h3a
      • HIST1H3B
      • HIST1H3C
      • HIST1H3D
      • HIST1H3E
      • HIST1H3F
      • HIST1H3G
      • HIST1H3H
      • HIST1H3I
      • HIST1H3J
      • HIST3H3
      • histone 1, H3a
      • Histone cluster 1, H3a
      • Histone H3 3 pseudogene
      • Histone H3.1
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/i
      • Histone H3/j
      • Histone H3/k
      • Histone H3/l
      see all
    • 功能

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 序列相似性

      Belongs to the histone H3 family.
    • 发展阶段

      Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
    • 翻译后修饰

      Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
      Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
      Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
      Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
      Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
      Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
    • 细胞定位

      Nucleus. Chromosome.
    • Target information above from: UniProt accession P68431 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    图片

    • Dot Blot - Human Histone H3 (tri methyl K9) peptide (ab1773)
      Dot Blot - Human Histone H3 (tri methyl K9) peptide (ab1773)This image is courtesy of an anonymous Abreview

      ab1773 (at 0.01 and 0.1 µg) used in Dot Blot to test for cross-reactivity of ab8898 (1/2500). An HRP-conjugated Mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.

      See Abreview

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (18)

    发表研究结果有使用 ab1773?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1773 被引用在 18 文献中.

    • Tang J  et al. Histone modifications associated with gene expression and genome accessibility are dynamically enriched at Plasmodium falciparum regulatory sequences. Epigenetics Chromatin 13:50 (2020). PubMed: 33225957
    • Ye S  et al. SET Domain-Containing Protein 4 Epigenetically Controls Breast Cancer Stem Cell Quiescence. Cancer Res 79:4729-4743 (2019). PubMed: 31308046
    • Poleshko A  et al. H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis. Elife 8:N/A (2019). PubMed: 31573510
    • Jia WH  et al. DEK terminates diapause by activation of quiescent cells in the crustacean Artemia. Biochem J 476:1753-1769 (2019). PubMed: 31189566
    • Zhong Y  et al. The novel methyltransferase SETD4 regulates TLR agonist-induced expression of cytokines through methylation of lysine 4 at histone 3 in macrophages. Mol Immunol 114:179-188 (2019). PubMed: 31376731
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Other (Dot Blot) Abreview for Histone H3 peptide - tri methyl K9

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Other - Do not use

    Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.
    Sample: Human Purified protein

    Primary antibody (in addition to 'Histone H3 peptide - tri methyl K9')
    Primary antibody: Abcam primary antibody: Anti-Histone H3 (methylated) antibody - ChIP Grade (ab8898)
    Dilution: 1/2500

    Secondary antibody
    Name: Non-Abcam antibody was used: anti-rabbit
    Host species: Mouse
    Clonality: Polyclonal
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/10000
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 01 2010

    Question


    I have ordered your mono-, di-, and tri-methyl H3K9 peptides (catalog) ab1771, ab1772 and ab1773, but I cant find sequence information on the website in terms of where the sequence starts and ends and how long they are.

    I need to order a control unmodified H3 peptide that matches these three modified peptides. I found several H3 peptides on your website. I would like to know which one to order so I get the peptide that starts and ends in the same positions as these modified peptides.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 18 2014

    Answer



    I would recommend using ab2903, a 14 aa peptide, as the corresponding unmodified peptide. It has 3 additional amino acids at the amino terminus of the peptide that are not included ab1771 and 2 additional amiino acids at the N-term as compared to ab1772 and ab1773.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    回复于 Aug 19 2014

    Question

    I am doing research with the model plant Arabidopsis. I can use the antibodies and peptides derived from human, because histone H3 is mostly conserved among many eukaryotes, however there are still differences at some amino acid positions. Therefore the information of the residue sequence or residue positions is very important for me to choose your products.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 15 2012

    Answer

    The sequence and purification method is propriety, but if you let me know what is important for you I can check if one of our product fits.

    Read More

    Abcam Scientific Support

    回复于 Oct 15 2012

    Question

    I’m looking to purchase peptides ab1773 and ab7768, however before I do I need to know their molecular masses to determine if we can visualise them.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 20 2012

    Answer

    The product ab1773 and ab7768 are very small peptides; due to their low molecular weight these products should not be used in SDS PAGE experiments.
    We have tested these product for blocking the antibody active sites so these products should be used in blocking experiments or in applications as recommended on the datasheet.

    Read More

    Abcam Scientific Support

    回复于 Jan 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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