为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Epigenetics and Nuclear Signaling Histones H3 Methylated

人Histone H3 (tri methyl K27)多肽(ab1782)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (10)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Dot Blot - Human Histone H3 (tri methyl K27) peptide (ab1782)

    Key features and details

    • Purity: >= 70% HPLC
    • Suitable for: Dot blot, Blocking

    你可能会感兴趣

    肽
    Product image
    Human Histone H3 (tri methyl K9) peptide (ab1773)
    一抗
    Product image
    Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    查看更多相关产品

    描述

    • 产品名称

      人Histone H3 (tri methyl K27)多肽
      参阅全部 Histone H3 蛋白酶
    • 纯度

      >= 70 % HPLC.
      70 - 90% by HPLC
    • Accession

      P68431
    • 无动物成分

      No
    • 性质

      Synthetic
      • 种属

        Human
      • 修饰

        tri methyl K27

    相关产品

    • Corresponding Antibody

      • Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)
      • Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)
    • Positive Controls

      • Human Histone H3 (di methyl K27) peptide (ab1781)

    技术指标

    Our Abpromise guarantee covers the use of ab1782 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Dot blot

      Blocking - Blocking peptide for Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002), Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (ab6147)

    • 形式

      Lyophilized
    • 补充说明

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at -20°C.

      Information available upon request.

    常规信息

    • 别名

      • H3 histone family member E pseudogene
      • H3 histone family, member A
      • H3/A
      • H31_HUMAN
      • H3F3
      • H3FA
      • Hist1h3a
      • HIST1H3B
      • HIST1H3C
      • HIST1H3D
      • HIST1H3E
      • HIST1H3F
      • HIST1H3G
      • HIST1H3H
      • HIST1H3I
      • HIST1H3J
      • HIST3H3
      • histone 1, H3a
      • Histone cluster 1, H3a
      • Histone H3 3 pseudogene
      • Histone H3.1
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/i
      • Histone H3/j
      • Histone H3/k
      • Histone H3/l
      see all
    • 功能

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 序列相似性

      Belongs to the histone H3 family.
    • 发展阶段

      Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
    • 翻译后修饰

      Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
      Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
      Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
      Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
      Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
      Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
    • 细胞定位

      Nucleus. Chromosome.
    • Target information above from: UniProt accession P68431 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    图片

    • Dot Blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
      Dot Blot - Human Histone H3 (tri methyl K27) peptide (ab1782)This image is courtesy of an anonymous Abreview

      ab1782 (at 0.01 and 0.1 μg) used in dot blot to test for cross-reactivity of an anti-H3K27me3 polyclonal antibody (1/2500). A HRP-conjugated mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.

      See Abreview

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (10)

    发表研究结果有使用 ab1782?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1782 被引用在 10 文献中.

    • Schwartz-Orbach L  et al. Caenorhabditis elegans nuclear RNAi factor SET-32 deposits the transgenerational histone modification, H3K23me3. Elife 9:N/A (2020). PubMed: 32804637
    • Kinkley S  et al. reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells. Nat Commun 7:12514 (2016). PubMed: 27530917
    • Maeng YS  et al. Histone methylation levels correlate with TGFBIp and extracellular matrix gene expression in normal and granular corneal dystrophy type 2 corneal fibroblasts. BMC Med Genomics 8:74 (2015). PubMed: 26553048
    • Hui SP  et al. Characterization of Proliferating Neural Progenitors after Spinal Cord Injury in Adult Zebrafish. PLoS One 10:e0143595 (2015). PubMed: 26630262
    • McLaughlin N  et al. In situ histone landscape of nephrogenesis. Epigenetics 9:222-35 (2014). PubMed: 24169366
    • Arambasic M  et al. Pdsg1 and Pdsg2, novel proteins involved in developmental genome remodelling in Paramecium. PLoS One 9:e112899 (2014). PubMed: 25397898
    • Huh YH & Sherley JL Decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells. Cell Death Dis 5:e1554 (2014). PubMed: 25476902
    • Wang S  et al. Lineage specific trimethylation of H3 on lysine 4 during C. elegans early embryogenesis. Dev Biol 355:227-38 (2011). PubMed: 21549110
    • McManus KJ  et al. Dynamic changes in histone H3 lysine 9 methylations: Identification of a mitosis-specific function for dynamic methylation in chromosome congression and segregation. J Biol Chem : (2005). PubMed: 16373353
    • Chadwick BP & Willard HF Multiple spatially distinct types of facultative heterochromatin on the human inactive X chromosome. Proc Natl Acad Sci U S A : (2004). PubMed: 15574503

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Other (Dot Blot) Abreview for Histone H3 peptide - tri methyl K27

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Other - Do not use

    Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.
    Sample: Human Purified protein

    Primary antibody (in addition to 'Histone H3 peptide - tri methyl K27')
    Name: Non-Abcam antibody was used: Anti-H3K27me3
    Host species: Rabbit
    Clonality: Polyclonal
    Dilution: 1/2500

    Secondary antibody
    Name: Non-Abcam antibody was used: Anti-rabbit
    Host species: Mouse
    Clonality: Polyclonal
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/10000
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 26 2010

    Question

    I am doing research with the model plant Arabidopsis. I can use the antibodies and peptides derived from human, because histone H3 is mostly conserved among many eukaryotes, however there are still differences at some amino acid positions. Therefore the information of the residue sequence or residue positions is very important for me to choose your products. In addition, the purity of the peptides is important to judge the quality of your products.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 15 2012

    Answer

    The sequence and purification method is propriety, but if you let me know what is important for you I can check if one of our product fits.

    Read More

    Abcam Scientific Support

    回复于 Oct 15 2012

    Question

    I am interested in doing some affinity purification of H3TrimK27 antibody to remove any antibodies that cross react with H3 trimethyl K9. Is there a way in which i can immobilize the H3TrimK27 peptide onto beads or are there sulfhybryl groups on the peptide?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 08 2005

    Answer

    The sequence contains a cysteine residue on the end which we use to conjugate to the matrix. The matrix which we use is called Sulfolink coupling gel from Perbio (20402B). Alternatively NHS activated sepharose can be used which links via the primary amino group to the NHS (N-hydroxysuccinimide). This is supplied by Amersham. I hope this makes sense but I would recommend the sulfolink as it is a quick protocol. I think it is recommended to use a 1mg/ml peptide solution for optimal peptide binding.

    Read More

    Abcam Scientific Support

    回复于 Jul 11 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有